{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:06:01Z","timestamp":1789657561757,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cryopreserved platelets can support availability in settings where fresh platelets are inaccessible. Freeze\u2010dried plasma (FDP) may serve as an alternative reconstitution medium to fresh frozen plasma (FFP) when plasma thawing is impractical. The effects of FDP on cryopreserved platelet quality remain underexplored. Thus, we aimed to evaluate its suitability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Cryopreserved platelets were prepared from double\u2010dose buffy\u2010coat concentrates, split, prewashed, and frozen using dimethyl sulfoxide (DMSO) (5\u20136%). After thawing, paired units were resuspended in FFP (AB\u2009=\u20098) or FDP (OctaplasLG Powder, AB\u2009=\u20098). Platelet count and mean platelet volume were analyzed alongside blood gas parameters. Additionally, extracellular lactate dehydrogenase (LDH), sP\u2010selectin, and VEGF were measured in the supernatant. Platelet markers (aggregation, adhesion, and activation) and microparticles were analyzed by flow cytometry. Clotting ability was evaluated using ROTEM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After thawing, FDP units contained significantly more platelets than FFP units 206\u2009\u00b1\u200927 versus 176\u2009\u00b1\u200918 \u00d710\n                      <jats:sup>9<\/jats:sup>\n                      \/units (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.0006). LDH activity was lower (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.040), whereas VEGF levels were higher (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.0003) in the FDP group. Oxygen and carbon dioxide pressure differed significantly, yet pH was normal. Phenotypic expression and microparticle content demonstrated no significant differences. FDP units showed a shorter clotting time in ROTEM EXTEM (50\u2009\u00b1\u20095 vs. 58\u2009\u00b1\u20099\u2009s,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.035), although clot strength was similar.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>FDP\u2010reconstituted platelets were functionally comparable to those reconstituted with FFP, while demonstrating improved recovery and enhanced clot initiation, potentially due to differences in plasma composition. Given its logistical advantages, particularly in resource\u2010limited settings, FDP represents a promising reconstitution medium for cryopreserved platelets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70129","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T07:20:49Z","timestamp":1771226449000},"page":"803-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improved platelet recovery in cryopreserved platelets reconstituted in freeze\u2010dried plasma"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4182-5826","authenticated-orcid":false,"given":"Kristina","family":"Ehn","sequence":"first","affiliation":[{"name":"Clinical Immunology and Transfusion Medicine Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Medicine Huddinge Karolinska Insitutet  Stockholm 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