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To address the need for high\u2010throughput genotyping, this study developed a method for simultaneous detection of 35 HPA systems, 5 HNA systems, and CD36 polymorphisms based on matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Specific primers for amplification and single\u2010base extension were designed, distributing 53 loci into two multiplex pools. Genotyping was performed using the MassARRAY MS platform. Method accuracy was verified by Sanger sequencing for all 53 loci (34 samples) and flow cytometric serotyping for HPA\u20101, \u20103, \u20105 and CD36. The validated method was then applied to genotype 8100 random blood donors to establish a platelet donor database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>An MS\u2010based genotyping method was successfully established, covering 53 loci distributed across two multiplex pools. All verified samples yielded results consistent with those obtained through Sanger sequencing, serotyping, or flow cytometry. Using this method, a platelet donor genotyping database comprising 8100 donors was created, identifying rare HPA alleles (1 case each of HPA\u20108b\/\u20109b\/\u201011b\/\u201016b\/\u201025b; 2 of HPA\u201017b\/\u201030b; 3 of HPA\u201020b).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>We successfully developed an MS panel capable of detecting all currently known HPAs, HNAs, and the CD36 antigen, overcoming limitations of previous methods related to non\u2010specific HNA\u20101 amplification. This method also demonstrated its efficacy in establishing a large\u2010scale platelet donor database, and rare allele data support precise transfusion matching for special patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70167","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:05:03Z","timestamp":1773378303000},"page":"998-1011","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of mass spectrometry\u2010based genotyping method for simultaneous detection of 35\n                    <scp>HPA<\/scp>\n                    systems, 5\n                    <scp>HNA<\/scp>\n                    systems and the\n                    <scp>CD36<\/scp>\n                    antigen"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-1476","authenticated-orcid":false,"given":"Huijun","family":"Zhu","sequence":"first","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruishu","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Fu","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qixiu","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Ma","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lu","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9407-7727","authenticated-orcid":false,"given":"Ziyan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyi","family":"Ye","sequence":"additional","affiliation":[{"name":"Shanghai Blood Center Institute of Blood Transfusion  Shanghai 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