{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:02:09Z","timestamp":1781071329456,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Patients with chronic transfusion needs such as those with sickle cell disease face a high risk of developing antibodies against high\u2010prevalence antigens in the RH blood group system, complicating transfusion therapy and potentially necessitating stem cell transplantation. Molecular characterization of the RH system is hindered by hybrid alleles and high sequence homology between\n                      <jats:italic>RHD<\/jats:italic>\n                      and\n                      <jats:italic>RHCE<\/jats:italic>\n                      , limiting the effectiveness of conventional short\u2010read sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>We analyzed 11 control and 20 patient samples, some of which could not be reliably genotyped by standard methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Nanopore sequencing with adaptive sampling enables targeted, amplification\u2010free long\u2010read sequencing of the\n                      <jats:italic>RH<\/jats:italic>\n                      locus, resolving homologous and complex hybrid structures and enabling complete haplotype phasing for all samples, including samples that could not be accurately determined by standard methods like serology and short\u2010read sequencing. Four new alleles were identified and for 13 out of 20 patients the results led to a change in the transfusion regimen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings show that nanopore sequencing with adaptive sampling allows unambiguous genotyping of the RH system, improves detection of complex variants, and supports better\u2010matched transfusion strategies for chronically transfused patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70182","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T05:22:34Z","timestamp":1775712154000},"page":"1058-1069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rhesus blood group haplotype determination by nanopore sequencing and adaptive sampling enables the precise determination of complex allele combinations that could not be accurately determined by standard methods"],"prefix":"10.1111","volume":"66","author":[{"given":"Rebekka","family":"Waldmann","sequence":"first","affiliation":[{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm German Red Cross Blood Service Baden\u2010W\u00fcrttemberg \u2013 Hessen and Ulm University Hospital  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita","family":"F\u00fchrer","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm German Red Cross Blood Service Baden\u2010W\u00fcrttemberg \u2013 Hessen and Ulm University Hospital  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Vogt","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm German Red Cross Blood Service Baden\u2010W\u00fcrttemberg \u2013 Hessen and Ulm University Hospital  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Nolde","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm German Red Cross Blood Service Baden\u2010W\u00fcrttemberg \u2013 Hessen and Ulm University Hospital  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Riecker","sequence":"additional","affiliation":[{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm German Red Cross Blood Service Baden\u2010W\u00fcrttemberg \u2013 Hessen and Ulm University Hospital  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart Finn","family":"Herrlich","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine Ulm University Hospital  Ulm 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