{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:02:20Z","timestamp":1781071340387,"version":"3.54.1"},"reference-count":11,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T00:00:00Z","timestamp":1775347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T00:00:00Z","timestamp":1775347200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Anti\u2010CD99 is a rare alloantibody with unclear clinical significance in transfusion medicine. We report a case of acute hemolytic transfusion reaction associated with anti\u2010CD99 in a female patient.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case Report<\/jats:title>\n                    <jats:p>The patient was a 74\u2010year\u2010old woman originally from Kyushu Prefecture with a history of pregnancy and transfusions. The patient was also diagnosed with Parkinson's disease 13\u2009years ago and recently hospitalized with intestinal obstruction and aspiration pneumonia. During hospitalization, she developed anemia requiring red blood cell transfusions; pre\u2010transfusion antibody screening before the second transfusion revealed pan\u2010reactivity with all panel cells. Antibody identification testing at the Japanese Red Cross Kyushu Block Blood Center detected anti\u2010CD99 in the patient's serum. As CD99\u2010negative blood was unavailable, two units of randomly selected red blood cells derived from male donors were transfused. Shortly after the second transfusion, the patient developed chills, fever, hematuria, and laboratory evidence of hemolysis. Flow cytometry and monocyte phagocytosis assays demonstrated that CD99 expression on donor red blood cells correlated with susceptibility to phagocytosis. The patient subsequently died on day 12 after the second transfusion due to worsening pneumonia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Antibody\u2010producing CD99\u2010negative individuals are extremely rare; in Japan, only 10 cases have been identified since 1993 at Japanese Red Cross blood centers. All of these patients were women from the Kyushu region. The present case demonstrates the potential of anti\u2010CD99 to cause acute hemolytic transfusion reactions and represents a valuable clinical report, emphasizing the importance of careful transfusion management in patients with anti\u2010CD99.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70206","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T06:45:52Z","timestamp":1775457952000},"page":"1219-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A case of acute hemolytic transfusion reaction caused by anti\u2010\n                    <scp>CD99<\/scp>"],"prefix":"10.1111","volume":"66","author":[{"given":"Masamu","family":"Sakoda","sequence":"first","affiliation":[{"name":"Japanese Red Cross Kyushu Block Blood Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kyushu Block Blood Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Kumamoto","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kyushu Block Blood Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Oda","sequence":"additional","affiliation":[{"name":"Fukuoka Prefectural Red Cross Blood Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Honda","sequence":"additional","affiliation":[{"name":"National Hospital Organization Fukuokahigashi Medical Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Tanaka","sequence":"additional","affiliation":[{"name":"National Hospital Organization Fukuokahigashi Medical Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Kuroiwa","sequence":"additional","affiliation":[{"name":"National Hospital Organization Fukuokahigashi Medical Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Naganuma","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumie","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-257X","authenticated-orcid":false,"given":"Naoko","family":"Watanabe\u2010Okochi","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatsue","family":"Tsuneyama","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kyushu Block Blood Center  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1987.tb01058.x"},{"key":"e_1_2_11_3_1","first-page":"23S","article-title":"An alloantibody to 12E7 antigen detected in 2 healthy donors","volume":"35","author":"Uchikawa M","year":"1995","journal-title":"Transfusion"},{"issue":"1","key":"e_1_2_11_4_1","first-page":"50","article-title":"Four examples of anti\u2010CD99 and discovery of the molecular bases of the rare CD99 phenotype","volume":"109","author":"Thornton NM","year":"2015","journal-title":"Vox Sang"},{"issue":"1","key":"e_1_2_11_5_1","first-page":"29","article-title":"An example of anti\u2010CD99 in an antenatal patient with a novel homozygous mutation in CD99, resulting in the rare CD99\u2010phenotype","volume":"116","author":"Borowski A","year":"2021","journal-title":"Vox Sang"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18126"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.5348\/100039Z02SI2018OA"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13425"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-03-842542"},{"key":"e_1_2_11_10_1","first-page":"1854","article-title":"The molecular genetic background leading to the formation of the human erythroid\u2010specific Xg(a)\/CD99 blood groups","volume":"2","author":"Yeh CC","year":"2018","journal-title":"Blood"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24265"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xgen.2023.100259"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70206","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70206","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:30:25Z","timestamp":1781069425000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,5]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/trf.70206"],"URL":"https:\/\/doi.org\/10.1111\/trf.70206","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,5]]},"assertion":[{"value":"2025-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}