{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:15:50Z","timestamp":1783653350604,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Chimeric antigen receptor (CAR) T\u2010cell therapy has transformed the treatment of hematologic malignancies, but its success depends on obtaining sufficient CD3\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell yields during leukapheresis. This can be difficult in heavily pretreated patients, who often show leukopenia and reduced T\u2010cell fitness.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed 166 leukapheresis products from 154 patients undergoing manufacturing of CD19\u2010directed CAR T\u2010cells, including 146 from non\u2010Hodgkin's lymphoma (NHL) and 20 from acute lymphoblastic leukemia (ALL). Collections were performed for commercial CAR T\u2010cell products (axi\u2010cel, tisa\u2010cel, brexu\u2010cel, liso\u2010cel;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009121) and the HD\u2010CAR\u20101 trial with in\u2010house manufacturing (heidagenlecleucel;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200945).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In 150\/154 patients, a sufficient\n                      <jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content>\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell yield was achieved by a single leukapheresis. Pre\u2010apheresis lymphocyte count strongly predicted\n                      <jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content>\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell yield (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; .001) and was associated with treatment response (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.044). Impaired hematopoiesis, reflected by reduced nucleated cell count (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; .001), lymphocyte count (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;.001), and hematocrit (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.017), was linked to poorer collection efficiency. Intensive prior therapies, including stem cell transplantation, reduced\n                      <jats:styled-content style=\"fixed-case\">CAR<\/jats:styled-content>\n                      T\u2010cell expansion during manufacturing (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.036). In patients with\n                      <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>\n                      , higher proportions of effector and\n                      <jats:styled-content style=\"fixed-case\">CD8<\/jats:styled-content>\n                      <jats:sup>+<\/jats:sup>\n                      <jats:styled-content style=\"fixed-case\">CAR<\/jats:styled-content>\n                      T\u2010cells in the product correlated with improved clinical outcomes (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.04;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.023).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings highlight the importance of early leukapheresis, ideally before intensive treatments, to optimize T\u2010cell yield, product quality, and therapeutic efficacy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70224","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:36:28Z","timestamp":1775889388000},"page":"1375-1389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impaired hematopoiesis affects apheresis and\n                    <scp>CAR<\/scp>\n                    T\u2010cell product composition and treatment response"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2281-858X","authenticated-orcid":false,"given":"Hanna","family":"Kuhn","sequence":"first","affiliation":[{"name":"Internal Medicine V, Hematology, Oncology and Rheumatology Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Korell","sequence":"additional","affiliation":[{"name":"Internal Medicine V, Hematology, Oncology and Rheumatology Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"H\u00fcckelhoven\u2010Krauss","sequence":"additional","affiliation":[{"name":"Internal Medicine V, Hematology, Oncology and Rheumatology Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Neuber","sequence":"additional","affiliation":[{"name":"Internal Medicine V, Hematology, Oncology and Rheumatology Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Stenzinger","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Cell Therapy (IKTZ)  Heidelberg Germany"},{"name":"Institute of Immunology University Hospital Heidelberg  Heidelberg 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