{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:16:54Z","timestamp":1783653414960,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Accurate prediction of peripheral blood stem cell (PBSC) yield is essential for optimizing hematopoietic stem cell collections. Traditional CD34+ prediction formulas often overlook the ongoing recruitment of CD34+ cells during the collection procedure. This study evaluated whether a single intra\u2010apheresis CD34+ measurement could improve yield prediction compared to existing prediction models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We retrospectively analyzed 150 PBSC collections from 123 autologous and allogenic donors. A single intraprocedural CD34\n                      <jats:sup>+<\/jats:sup>\n                      measurement and final collection yield were obtained, and univariable and multivariable linear regressions were developed to assess predictive accuracy. Model stability and generalizability were tested via bootstrap resampling and cross\u2010validation. Subgroup analyses by age, sex, weight, diagnosis, and sampling time were performed to confirm robustness. We also compared our results against published pre\u2010apheresis formulas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intra\u2010apheresis CD34\n                      <jats:sup>+<\/jats:sup>\n                      counts showed a strong correlation with the final yield (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.97;\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.95). Subgroup analyses across donor demographics and clinical factors yielded similarly high\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      values (0.91\u20130.99). Multivariable analysis identified intra\u2010apheresis CD34+ measurement as the primary contributor (\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2009=\u20090.67,\n                      <jats:italic>p<\/jats:italic>\n                      &lt;.0001). Later sampling (\u22651.20 of total blood volumes processed) reduced error metrics relative to earlier draws. Compared to pre\u2010apheresis models, the intra\u2010apheresis approach achieved higher\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      and lower error metrics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>A single intra\u2010apheresis CD34+ measurement, particularly at or beyond 1.20 processed blood volumes, offers a reliable, real\u2010time predictor of final PBSC yield. This intraprocedural approach outperforms published pre\u2010apheresis formulas, enabling more efficient collection and reduced donor burden across diverse clinical scenarios.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70225","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T11:39:13Z","timestamp":1776944353000},"page":"1390-1400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intra\u2010apheresis\n                    <scp>CD34<\/scp>\n                    + cell count: A dynamic approach to predicting peripheral blood stem cell collection yield"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0815-3170","authenticated-orcid":false,"given":"Abdullah","family":"Alswied","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mame","family":"Thioye\u2010Sall","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard N.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8152-804X","authenticated-orcid":false,"given":"Kamille","family":"West\u2010Mitchell","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5867-3265","authenticated-orcid":false,"given":"David","family":"Stroncek","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9715-7743","authenticated-orcid":false,"given":"Anh","family":"Dinh","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Center for Cellular Engineering, Clinical Center National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-208355"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04364.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.02.005"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp2314279"},{"key":"e_1_2_10_6_1","unstructured":"Incorporated VP.CASGEVY (exagamglogene autotemcel) [package insert]. 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