{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:04:08Z","timestamp":1781071448913,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","award":["BEAP202001\u2010JA"],"award-info":[{"award-number":["BEAP202001\u2010JA"]}],"id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cryopreserved red cell concentrates (RCCs) glycerolized using a high glycerol (40%) method can be stored below \u221265\u00b0C for up to 30\u2009years; however, units may be inadvertently warmed above \u221265\u00b0C due to freezer failures, human errors, or routine inventory management. RCCs known to have experienced transient warming events (TWEs) or storage temperatures \u2265\u221265\u00b0C are frequently discarded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>\n                      Six groups of ABO\/Rh matched RCCs were pooled\u2010and\u2010split (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20099), glycerolized using the ACP 215 (Haemonetics), and frozen. Three types of TWEs were performed: (1) \u201cFast\u201d TWEs (warming at room temperature [RT] to \u221225\u00b0C), (2) \u201cSlow\u201d TWEs (exposure to \u221225\u00b0C for 2\u2009h), and (3) \u201cThaw\u201d TWEs (single warming to RT). Units (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096) were exposed to 0 (control), 1, 10, or 30 \u201cSlow\u201d or \u201cFast\u201d TWEs. RCCs were deglycerolized using the ACP 215, stored at 1\u20136\u00b0C, and tested at 0, 1, 7, and 14\u2009days post\u2010deglycerolization for red blood cell (RBC) quality.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>No significant differences among RCCs over hypothermic storage were found when exposed to \u201cFast\u201d or \u201cSlow\u201d single or \u201cThaw\u201d TWEs, although a significant decrease in the quality of RCCs was observed when units were subjected to 10 or more \u201cFast\u201d or \u201cSlow\u201d TWEs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our data suggests the single TWEs examined in this study do not significantly impact the quality of RCCs post\u2010deglycerolization. Based on these observations, TWE exposure considerations should be made on a unit\u2010by\u2010unit basis, with additional policy support for unit retention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70253","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T06:31:49Z","timestamp":1777962709000},"page":"1180-1191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hold on to your units: Quality of red cell concentrates is only affected after multiple transient warming events"],"prefix":"10.1111","volume":"66","author":[{"given":"Jayme","family":"Kurach","sequence":"first","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Brandon\u2010Coatham","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5299-9763","authenticated-orcid":false,"given":"Nishaka","family":"William","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1192-4717","authenticated-orcid":false,"given":"Carly","family":"Olafson","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey R.","family":"Turner","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8854-6750","authenticated-orcid":false,"given":"Anika Tahsin","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behrouz","family":"Ehsani\u2010Moghaddam","sequence":"additional","affiliation":[{"name":"The Southeastern Ontario Academic Medical Organization, Faculty of Health Sciences Queen's University  Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwen","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1445-827X","authenticated-orcid":false,"given":"Jason P.","family":"Acker","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2021.01.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18054"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15027"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2020.102853"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1363.010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.3.C125"},{"key":"e_1_2_10_8_1","article-title":"Recrystallization revisited","volume":"7","author":"Forsyth M","year":"1986","journal-title":"Cryoletters"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23619"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12080"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16017"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-373-9:049"},{"issue":"5","key":"e_1_2_10_13_1","first-page":"426","article-title":"Performance characteristics of Hemox\u2010analyzer for assessment of the hemoglobin dissociation curve","volume":"80","author":"Guarnone R","year":"1995","journal-title":"Haematologica"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12679"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.49.4.271"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1975.tb02756.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017004820"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000206398"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-2010-1384"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365513.2014.993695"},{"key":"e_1_2_10_21_1","unstructured":"CSA Group.Blood and blood components. CAN\/CSA\u2010Z902:20.2020."},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41030401.x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2024.104903"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17749"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13602"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17138"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18088"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12112"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13147"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12194"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12746"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01697397"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12296"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21733"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(69)90287-3"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.443501"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00692a032"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/b901737b"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(73)90023-0"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2015.0111"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.8b02126"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0001-6160(58)90020-8"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70253","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:29:52Z","timestamp":1781069392000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,4]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/trf.70253"],"URL":"https:\/\/doi.org\/10.1111\/trf.70253","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,4]]},"assertion":[{"value":"2026-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}