{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:16:07Z","timestamp":1789168567000,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["75D30120C08170"],"award-info":[{"award-number":["75D30120C08170"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015302","name":"Ortho Clinical Diagnostics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015302","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The two largest US blood collection organizations led a multiphased, multicenter nationwide SARS\u2010CoV\u20102 blood donor\u2010based serosurveillance program. The potential impact of delayed sample processing and prolonged refrigerated or frozen storage on anti\u2010SARS\u2010CoV\u20102 antibody detection was investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Anti\u2010spike (S) IgG and anti\u2010nucleocapsid (N) total immunoglobulin levels were compared for 20 paired plasma\/serum samples tested fresh and frozen after separation from cellular components on Day 5 or after \u226440\u2009days of 4\u00b0C storage; 10 paired plasma\/serum samples separated on Day 5 and tested immediately or after \u226467\u2009days of 4\u00b0C storage; and 85 plasma and 950 serum samples tested fresh on Day 1 and after \u2264209\u2009days of \u221220\u00b0C storage post\u2010separation. Proportional Bland\u2013Altman analyses were used to estimate average bias and 95% limits of agreement (LOA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Antibody reactivity was stable over time regardless of serum or plasma processing delays or prolonged refrigerated or frozen storage. Bland\u2013Altman analyses suggest only minor deviations for anti\u2010S antibodies for samples separated within 5\u2009days and tested frozen compared to fresh (bias: 4.23%, 95% LOA: \u221210.02; 18.47), after \u226440\u2009days of storage at 4\u00b0C pre\u2010separation (9.51%, [\u221273.20; 92.22]) and after \u226467\u2009days at 4\u00b0C post\u2010separation (\u221214.04%, [\u221228.12; 0.04]). Similar observations were obtained for anti\u2010N antibodies including samples retested after \u2264209\u2009days of storage at \u221220\u00b0C (\u221220.3%, [\u221281; 40.5]).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Despite challenges encountered in sample access and processing during a rapidly evolving pandemic, sample processing variability and testing delays minimally impacted serologic results and thus provide evidence for the validity of results in studies requiring variable sample handling conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70283","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T11:19:40Z","timestamp":1781090380000},"page":"1800-1810","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of delayed blood donation sample processing and testing on\n                    <scp>SARS<\/scp>\n                    \u2010\n                    <scp>CoV<\/scp>\n                    \u20102 serological assay results in an evolving pandemic response"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0855-7487","authenticated-orcid":false,"given":"Marion C.","family":"Lanteri","sequence":"first","affiliation":[{"name":"Creative Testing Solutions  Tempe Arizona USA"},{"name":"University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Green","sequence":"additional","affiliation":[{"name":"Creative Testing Solutions  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan","family":"Sulaeman","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mars","family":"Stone","sequence":"additional","affiliation":[{"name":"University of California San Francisco  San Francisco California USA"},{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Haynes","sequence":"additional","affiliation":[{"name":"American Red Cross  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Di Germanio","sequence":"additional","affiliation":[{"name":"University of California San Francisco  San Francisco California USA"},{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakina","family":"Smith","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia G.","family":"Villaflor","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamel","family":"Groves","sequence":"additional","affiliation":[{"name":"American Red Cross  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip C.","family":"Williamson","sequence":"additional","affiliation":[{"name":"Creative Testing Solutions  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Stramer","sequence":"additional","affiliation":[{"name":"Infectious Disease Consultant  North Potomac Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M.","family":"Coughlin","sequence":"additional","affiliation":[{"name":"National Center for Immunization and Respiratory Diseases, CDC  Atlanta 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