{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:15:54Z","timestamp":1789168554365,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K19198"],"award-info":[{"award-number":["24K19198"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Granulocyte colony\u2010stimulating factor (G\u2010CSF)\u2010based mobilization is essential for peripheral blood stem cell (PBSC) collection for allogeneic transplantation. Conventional daily G\u2010CSF requires repeated dosing and dose adjustment and often necessitates leukapheresis over multiple consecutive days, increasing donor burden. Single\u2010dose pegfilgrastim has emerged as an alternative; however, real\u2010world data on its impact on CD34\n                      <jats:sup>+<\/jats:sup>\n                      cell collection in healthy donors remain limited.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      Healthy related or unrelated donors who received either a single dose of pegfilgrastim or daily filgrastim and subsequently underwent PBSC collection at Kyoto University Hospital between July 2014 and September 2025 were included. CD34\n                      <jats:sup>+<\/jats:sup>\n                      cell harvest outcomes and donor burden were compared.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 39 donors were analyzed (20 pegfilgrastim, 19 filgrastim), with comparable baseline characteristics. At the start of leukapheresis, peripheral blood white blood cell counts were significantly higher in the pegfilgrastim group. Pegfilgrastim achieved greater total CD34\n                      <jats:sup>+<\/jats:sup>\n                      cell yield (median 398.0\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      vs. 302.4\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      cells,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.025) and higher yields per 10\u2009L of processed blood (median 407.8\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      vs. 302.4\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      cells,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.032). Single\u2010session leukapheresis was more frequent with pegfilgrastim (95.0% vs. 63.2%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.002), and the estimated processing time required to collect 2\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      \/kg CD34\n                      <jats:sup>+<\/jats:sup>\n                      cells was shorter (median 62.7 vs. 111.1\u2009min,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.040). No severe adverse events were observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings suggest that single\u2010dose pegfilgrastim enables more efficient PBSC collection with reduced donor burden and may represent a practical mobilization strategy in healthy allogeneic donors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70286","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T09:21:51Z","timestamp":1780046511000},"page":"1811-1818","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Usefulness of pegfilgrastim for allogeneic peripheral blood stem cell collection from healthy donors"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7690-8690","authenticated-orcid":false,"given":"Tomoshige","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-0421","authenticated-orcid":false,"given":"Tomoyasu","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"},{"name":"Department of Cytotherapy Kyoto University Hospital  Kyoto Japan"},{"name":"Center for Research and Application of Cellular Therapy Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6508-9432","authenticated-orcid":false,"given":"Noriyoshi","family":"Yoshinaga","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Matsui","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kitawaki","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Kanda","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoko","family":"Nishikori","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"},{"name":"Department of Human Health Sciences, Graduate School of Medicine Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Nagao","sequence":"additional","affiliation":[{"name":"Center for Research and Application of Cellular Therapy Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akifumi","family":"Takaori\u2010Kondo","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9662-5093","authenticated-orcid":false,"given":"Yasuyuki","family":"Arai","sequence":"additional","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"},{"name":"Department of Cytotherapy Kyoto University Hospital  Kyoto Japan"},{"name":"Center for Research and Application of Cellular Therapy Kyoto University Hospital  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)92009-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-021-01227-8"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtct.2025.05.014"},{"key":"e_1_2_11_5_1","unstructured":"Japanese Data Center for Hematopoietic Cell Transplantation (JDCHCT).Hematopoietic Cell Transplantation in Japan 2024: Summary Slides [Internet].2024[accessed 2025 Dec 22]. 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