{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T18:50:35Z","timestamp":1785178235788,"version":"3.55.0"},"reference-count":6,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Allogeneic hematopoietic stem cell transplantation (HSCT) is a treatment for various hematopoietic diseases. ABO incompatibility occurs in approximately 30%\u201340% of allogeneic HSCTs and is classified as major, minor, or bidirectional. A significant risk of ABO\u2010incompatible transplants is immediate hemolysis, which is why RBC depletion is the gold standard for avoiding this complication. Gelofusine, a colloidal solution based on succinyl gelatin, was recently validated by our laboratory as a washing solution for cryopreserved hematopoietic stem cells (HSCs) (Nappo et al.,\n                      <jats:italic>Transfusion<\/jats:italic>\n                      2025; 65(6):1101\u20131110). Our study aims to evaluate Gelofusine as a novel, rapid, and manual method for RBC depletion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>The sedimentation procedure was performed on 30\u2009units of cord blood considered unsuitable for transplantation; colloidal solution is added in a stoichiometric ratio. The mixture was gently mixed in sterile conditions and sedimentation occurred at room temperature. The process was carried out at different times until the complete sedimentation in two different components: erythrocytes and supernatant. For each unit, leukocyte recovery (WBCs recovery) and erythrocyte depletion (RBCs depletion) were calculated. Statistical analysis was performed using a one\u2010way ANOVA test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      WBCs recovery was 79%\u2009\u00b1\u200912, and RBCs depletion was 90%\u2009\u00b1\u20095. Sedimentation time statistically significantly influenced WBCs recovery (\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value \u2264.02) but not RBCs depletion. Comparison with HES solution has shown a significantly different (79 vs. 66)% regarding WBC recovery.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Gelofusine represents a valid alternative to automated systems for carrying out erythrodepletion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70289","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T10:05:56Z","timestamp":1781085956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gelofusine: A new tool for Erythrosedimentation"],"prefix":"10.1111","author":[{"given":"Mario","family":"Toriello","sequence":"first","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Nappo","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Lama","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Topo","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Gallo","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Castellano","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Esposito","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Auriemma","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Martino","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Rossitti","sequence":"additional","affiliation":[{"name":"Stem Cell Transplantation and Cell Therapy Unit AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9145-3691","authenticated-orcid":false,"given":"Francesco Paolo","family":"Tambaro","sequence":"additional","affiliation":[{"name":"Stem Cell Transplantation and Cell Therapy Unit AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Penta de Vera","family":"d'Aragona","sequence":"additional","affiliation":[{"name":"Ba.S.C.O., Cell Processing and Immunogenetics Unit, Oncology, Hematology and Cellular Therapy Department AORN Santobono\u2010Pausilipon  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18272"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1985.tb00152.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2450\/2016.0057-16"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2022.12.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2023.07.010"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.1998.00171.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70289","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70289","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T10:05:57Z","timestamp":1781085957000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,10]]},"references-count":6,"alternative-id":["10.1111\/trf.70289"],"URL":"https:\/\/doi.org\/10.1111\/trf.70289","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,10]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"trf.70289"}}