{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:16:14Z","timestamp":1789168574658,"version":"build-2803163510"},"reference-count":8,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,9]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Interpretation of antibody identification panels remains a manual, expertise\u2010intensive step in transfusion testing; interpretive errors and long turnaround times persist even in well\u2010equipped hospitals. Existing commercial software is closed source and often omits enzyme\u2010phase, cold\u2010phase, and dosage\u2010sensitive exclusion rules, limiting third\u2010party verification and local adaptation.<\/jats:p>\n <\/jats:sec>\n \n Study Design and Methods<\/jats:title>\n We developed IrregulAB, an open\u2010source (LGPL) web application that encodes serologic decision rules in two stages. First, a rule\u2010based screening stage applies LISS, enzyme, and cold rule\u2010out logic with safeguards for antigen dosage in heterozygotes to discard antibodies incompatible with the observed reaction pattern. Second, a minimal\u2010set solver exhaustively searches for the smallest antibody combination(s) that reproduce all assessable reactions and reports all equally plausible solutions. The system was evaluated on 108 development antibody\u2010identification panels and 35 held\u2010out temporal validation panels.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The screening stage retained the complete reference antibody set in 93% of development panels and 91% of held\u2010out temporal validation panels. The minimal\u2010set stage produced at least one valid explanatory antibody combination in 87% of development panels and 89% of held\u2010out temporal validation panels.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n IrregulAB is a transparent, rule\u2010based decision\u2010support tool for trained immunohematology personnel; it is not an autonomous antibody\u2010identification or reporting system. By exposing every encoded decision rule, it enables independent verification, local tuning, and more standardized review of routine antibody workups under expert supervision. Its current performance was evaluated retrospectively in a single\u2010center Bio\u2010Rad gel\u2010card dataset; multicenter and multi\u2010platform validation is required before wide clinical implementation.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.70301","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:32:25Z","timestamp":1781796745000},"page":"1758-1767","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IrregulAB<\/scp>\n : An open\u2010source rule\u2010based engine for automated red cell antibody panel interpretation"],"prefix":"10.1111","volume":"66","author":[{"given":"Davide","family":"Crucitti","sequence":"first","affiliation":[{"name":"Health Research Institute of Santiago de Compostela Santiago de Compostela Spain"},{"name":"University of Santiago de Compostela Faculty of Pharmacy Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta Vidal","family":"Ballester","sequence":"additional","affiliation":[{"name":"Hospital Universitari Vall d'Hebron Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas G\u00f3mez","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Health Research Institute of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia Fernandez","family":"Mellid","sequence":"additional","affiliation":[{"name":"Complejo Hospitalario Universitario de Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angie Lizeth Ruge","family":"Perdomo","sequence":"additional","affiliation":[{"name":"Complejo Hospitalario Universitario de Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7972-9431","authenticated-orcid":false,"given":"Carlos P\u00e9rez","family":"M\u00edguez","sequence":"additional","affiliation":[{"name":"Health Research Institute of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-6750","authenticated-orcid":false,"given":"Adri\u00e1n Mosquera","family":"Orgueira","sequence":"additional","affiliation":[{"name":"Health Research Institute of Santiago de Compostela Santiago de Compostela Spain"},{"name":"Complejo Hospitalario Universitario de Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16246"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37397240212.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00996197"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1987.27187121480.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-68716-5_41"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2014.08.020"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx149.439"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2018.10.022"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70301","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:51:23Z","timestamp":1789167083000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":8,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/trf.70301"],"URL":"https:\/\/doi.org\/10.1111\/trf.70301","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,18]]},"assertion":[{"value":"2026-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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