{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T12:18:34Z","timestamp":1783081114579,"version":"3.54.6"},"reference-count":16,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Centralization of blood donor screening and implementation of fully automated testing platforms have increased sample transport distances. Although transport is usually performed under controlled conditions (2\u20138\u00b0C), temperature excursions may occur. This study investigated the impact of such deviations on blood donor screening using a controlled preanalytical approach.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      Whole blood samples (EDTA\u2010K3, citrate plasma, and serum tubes) were collected from 10 regular plasma donors per temperature condition (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200930) and stored at 4, 22, or \u221210\u00b0C. To assess the impact of temperature excursions on analytical sensitivity, samples were supplemented with positive material representing low\u2010level analytes relevant for blood donor screening, including infectious serology and quantitative parameters. Samples were analyzed for key transfusion\u2010relevant markers for up to 8\u2009days post\u2010collection using CE\u2010certified automated systems from Roche, Beckman Coulter, and Werfen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both qualitative (infectious serology, nucleic acid amplification testing, blood group) and quantitative parameters (e.g., immunoglobulin G and total protein) remained stable across all temperatures and time points. Analytical sensitivity and specificity for blood donor screening assays were consistently 100%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>High analytical stability was demonstrated across a broad temperature range (\u221210 to 22\u00b0C), simulating extreme transport conditions. Reliable blood donor screening was achievable even after up to 8\u2009days of storage without immediate centrifugation. These findings suggest that moderate temperature excursions during transport may not compromise screening results and that extending acceptable preanalytical conditions could optimize logistics, reduce sample discard, and support continuous availability of safe blood products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70308","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:25:13Z","timestamp":1783077913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the preanalytical stability of blood donor samples"],"prefix":"10.1111","author":[{"given":"Kai","family":"Hourfar","sequence":"first","affiliation":[{"name":"Blood Donor Screening DRK Blutspendedienst Baden\u2010W\u00fcrttemberg\u2014Hessen gGmbH  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Xia","sequence":"additional","affiliation":[{"name":"Blood Donor Screening DRK Blutspendedienst Baden\u2010W\u00fcrttemberg\u2014Hessen gGmbH  Frankfurt Germany"},{"name":"Blood Donor Screening Octapharma Plasma GmbH  Langenfeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Mainzer","sequence":"additional","affiliation":[{"name":"Blood Donor Screening Octapharma Plasma GmbH  Langenfeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Peilst\u00f6cker","sequence":"additional","affiliation":[{"name":"Blood Donor Screening DRK Blutspendedienst Baden\u2010W\u00fcrttemberg\u2014Hessen gGmbH  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Prothmann","sequence":"additional","affiliation":[{"name":"Blood Donor Screening DRK Blutspendedienst Baden\u2010W\u00fcrttemberg\u2014Hessen gGmbH  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-0900","authenticated-orcid":false,"given":"Michael","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Blood Donor Screening DRK Blutspendedienst Baden\u2010W\u00fcrttemberg\u2014Hessen gGmbH  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Taborski","sequence":"additional","affiliation":[{"name":"Blood Donor Screening Octapharma Plasma GmbH  Langenfeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"issue":"2","key":"e_1_2_9_2_1","first-page":"209","article-title":"Safety of blood and blood products: test methods for the detection of hepatitis B, C, and E virus","volume":"65","author":"Mitterreiter J","year":"2022","journal-title":"Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz"},{"issue":"2","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1159\/000529872","article-title":"How to ensure blood supply and blood safety in the future","volume":"50","author":"Schlenke P","year":"2023","journal-title":"Transfus Med Hemother"},{"issue":"11","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1515\/cclm-2018-1146","article-title":"Collection, transport and storage procedures for blood culture specimens in adult patients: recommendations from a board of Italian experts","volume":"57","author":"Plato F","year":"2019","journal-title":"Clin Chem Lab Med"},{"key":"e_1_2_9_5_1","unstructured":"AramburuE RabasaP EsquirozR GalarretaT OlcozB.Valoracion de un antisuero anti\u2010D IgM\u2010IgG monoclonal (DIAGAST) en donantes de sangre con expresividad debil del antigeno D.Hematology Congress Madrid.1990."},{"issue":"2","key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1080\/14737159.2017.1275962","article-title":"The cobas\u00ae 6800\/8800 system: a new era of automation in molecular diagnostics","volume":"17","author":"Cobb B","year":"2017","journal-title":"Expert Rev Mol Diagn"},{"issue":"2","key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1093\/biostatistics\/kxp050","article-title":"Confidence intervals that match Fisher's exact or Blaker's exact tests","volume":"11","author":"Fay MP","year":"2010","journal-title":"Biostatistics"},{"issue":"5","key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3390\/diagnostics10050334","article-title":"Reporting standards for a Bland\u2010Altman agreement analysis: a review of methodological reviews","volume":"10","author":"Gerke O","year":"2020","journal-title":"Diagnostics"},{"key":"e_1_2_9_9_1","unstructured":"BeinG BenderA GreinacherA HilgerA KasperR Kl\u00fcterH et al.Guideline for the collection of blood and blood components and the use of blood products (Guideline on Hemotherapy): complete revision 2023. Berlin: German Medical Association; 2023. Official announcement of the German Medical Association dated 2023 Aug 29. Federal Gazette. 2023 Oct 26;BAnz AT 26.10.2023 B5."},{"issue":"2","key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1159\/000526761","article-title":"Analysis of transfusion\u2010transmitted bacterial infections according to German hemovigilance data (2011\u20102020)","volume":"50","author":"Orru' S","year":"2023","journal-title":"Transfus Med Hemother"},{"issue":"5","key":"e_1_2_9_11_1","first-page":"1","article-title":"Universal detection of mi(a) antigen and frequencies of glycophorin hybrids among blood donors in Taiwan by human monoclonal antibodies against mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) antigens","volume":"11","author":"Yang M\u2010H","year":"2021","journal-title":"Diagnostics"},{"issue":"4","key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1177\/0956462414542987","article-title":"Probability of a false\u2010negative HIV antibody test result during the window period: a tool for pre\u2010 and post\u2010test counselling","volume":"26","author":"Taylor D","year":"2015","journal-title":"Int J STD AIDS"},{"issue":"2","key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10561-023-10106-z","article-title":"Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials","volume":"25","author":"Padalko E","year":"2024","journal-title":"Cell Tissue Bank"},{"issue":"1","key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1515\/cclm-2021-0866","article-title":"Total lab automation: sample stability of clinical chemistry parameters in an automated storage and retrieval module","volume":"60","author":"Vercruysse K","year":"2022","journal-title":"Clin Chem Lab Med"},{"issue":"8","key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1136\/jcp-2022-208649","article-title":"Sample suitability and stability in different blood collection tubes for methanol, ethanol, isopropanol, acetone and glycols analysis","volume":"77","author":"Cui L","year":"2024","journal-title":"J Clin Pathol"},{"issue":"6","key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.clinbiochem.2012.01.012","article-title":"Stability study of 81 analytes in human whole blood, in serum and in plasma","volume":"45","author":"Oddoze C","year":"2012","journal-title":"Clin Biochem"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.cca.2024.120035","article-title":"Pre\u2010processing stability of routine clinical chemistry analytes in a clotting tube; investigating the (un)suitability for at\u2010home sample collection","volume":"567","author":"Roelofs M","year":"2025","journal-title":"Clin Chim Acta"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70308","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70308","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:25:33Z","timestamp":1783077933000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,3]]},"references-count":16,"alternative-id":["10.1111\/trf.70308"],"URL":"https:\/\/doi.org\/10.1111\/trf.70308","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,3]]},"assertion":[{"value":"2025-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"trf.70308"}}