{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:43:57Z","timestamp":1792158237897,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015539","name":"Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015539","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To maximize the utilization of cold\u2010stored platelets (CSPs) in preclinical research, the use of small\u2010volume storage bags (mini\u2010bags) or tubes would allow multiple treatment groups to be investigated from a single component. It is necessary to determine the effect of using downscaled storage formats on CSP quality and function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mini\u2010bags were prepared by vacuum sealing platelet storage bags to \u00bc the standard size. Apheresis platelets (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) in 40% plasma\/60% PAS\u2010E (SSP+) were aseptically aliquoted into mini\u2010bags (30\u2009mL) or polypropylene tubes (15\u2009mL; CSP\u2010tubes), and the remaining volume (~190\u2009mL) was retained in the original bag (control). Platelets were refrigerated (2\u20136\u00b0C), and in vitro quality parameters were assessed over 21\u2009days.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Metabolic capacity was altered for CSP\u2010tubes, with a significant decline in pH, mitochondrial membrane depolarization, and rapid glycolysis. CSP\u2010tubes had greater storage\u2010related activation, as evidenced by a higher proportion of annexin\u2010V\u2010positive platelets and a greater number of CD61+\/annexin\u2010V+ extracellular vesicles (EVs). Similarly, platelet functional capacity in CSP\u2010tubes was compromised, with reduced clot strength compared to controls. CSPs stored in mini\u2010bags remained comparable to controls, with the exception of a greater proportion of annexin\u2010V\u2010positive platelets and EVs at day 21. Importantly, despite these changes, no functional deficits were observed in mini\u2010bag components.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Storage in tubes markedly impairs the metabolic, structural, and functional quality of CSPs, indicating that this approach is unsuitable. In comparison, mini\u2010bag storage preserves key in vitro parameters, supporting a scalable strategy that maximizes the utilization of CSPs for preclinical evaluation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70339","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T06:20:01Z","timestamp":1786083601000},"page":"1952-1962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Downscaling to small\u2010volume storage bags supports platelet quality and efficient use of cold\u2010stored platelets for preclinical studies"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7801-388X","authenticated-orcid":false,"given":"Janhavi","family":"Mahajan","sequence":"first","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Alexandria New South Wales Australia"},{"name":"School of Life Sciences, University of Technology Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P.","family":"Padula","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3674-6934","authenticated-orcid":false,"given":"Denese C.","family":"Marks","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Alexandria New South Wales Australia"},{"name":"Sydney Medical School, University of Sydney  Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3303-4437","authenticated-orcid":false,"given":"Lacey","family":"Johnson","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Alexandria New South Wales Australia"},{"name":"School of Life Sciences, University of Technology Sydney  Sydney New South Wales Australia"},{"name":"School of Science, RMIT University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Guide to the preparation, use and quality assurance of blood components","author":"Council of Europe","year":"2025"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2018.04.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15255"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00803.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13630"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15651"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtha.2023.11.025"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15150"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15669"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16489"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16530"},{"key":"e_1_2_10_13_1","unstructured":"U.S. Food and Drug Administration Center for Biologcs Evaluation and Research.Alternative procedures for the manufacture of cold\u2010stored platelets intended for the treatment of active bleeding when conventional platelets are not available or their use is not practical 21 CFR 640.120(b).Maryland:Food and Drug Administration;2023. 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