{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T12:32:49Z","timestamp":1794400369861,"version":"4.9.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      In patients with variant RhD phenotypes, serologic reactions for partial\n                      <jats:italic>RHD<\/jats:italic>\n                      genotypes are indistinguishable from other variants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      In two US transfusion services, patients were typed for RhD by automated\n                      <jats:italic>microplate RhD typing (Werfen,<\/jats:italic>\n                      Norcross, GA) using two directly agglutinating IgM anti\u2010D reagents, MS201 (epitope RhD (epD) 6.1) in Series 4 (Anti\u2010D4) and TH\u201028 (epD6.4) in Series 5 (Anti\u2010D5). For weak or discrepant RhD typing, we performed a\n                      <jats:italic>RHD<\/jats:italic>\n                      DNA microarray (RHD BeadChip, Werfen, Norcross, GA). For hemi\u2010 or homozygous c.697G&gt;A (rs1053359), we obtained c.1136C&gt;T (rs61740966) testing (New York Blood Center) to distinguish DV type 5 (DV.5) from DAU4 (c.1136T). We retrospectively examined previous cases with negative\u2010weak anti\u2010D4 and strong anti\u2010D5 reactions for correlation with either DV.5 or DAU4 genotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Eight of 442 patients with weak or discrepant RhD phenotypes (1.8%) had 0\u20131+ anti\u2010D4, 3\u20134+ anti\u2010D5 phenotypes. They were the only 8 patients with c.697G&gt;A (correlation\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001, Fisher's exact test). Two had DV.5 (Hispanic, Armenian), 5 had DAU4 (4 Black, 1 Other), and 1 was not tested for c.1136 (Black). None had anti\u2010D.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      DV.5 and DAU4 alleles express disruptive p.Glu233Lys and are associated with hemolytic anti\u2010D.\n                      <jats:italic>Microplate RhD typing<\/jats:italic>\n                      with decreased IgM anti\u2010D4 reactions did not associate DV.5 and DAU4 with alloimmunization but indicated increased risk of anti\u2010D alloimmunization. Similar epD6.1\u2010weak, epD6.4\u2010strong RhD reactions were observed in 10 DV.5 patients using automated microcolumn typing. Development of anti\u2010D reagents detecting patients with other partial\n                      <jats:italic>RHD<\/jats:italic>\n                      genotypes beyond DVI is desirable.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70408","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:46:15Z","timestamp":1791006375000},"page":"2116-2122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A distinctive\n                    <scp>RhD<\/scp>\n                    serologic phenotype expressed by p.\n                    <scp>Glu233Lys RHD<\/scp>\n                    alleles\n                    <scp>DV<\/scp>\n                    type 5 and\n                    <scp>DAU4<\/scp>\n                    in microplate agglutination\n                    <scp>RhD<\/scp>\n                    typing"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5227-8104","authenticated-orcid":false,"given":"Vidyadhari","family":"Karne","sequence":"first","affiliation":[{"name":"Department of Pathology Transfusion Medicine, Los Angeles General Medical Center\/University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Jedrzejczak","sequence":"additional","affiliation":[{"name":"Department of Pathology Feinberg School of Medicine, Northwestern University, and Blood Bank, Northwestern Memorial Hospital  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul F.","family":"Lindholm","sequence":"additional","affiliation":[{"name":"Department of Pathology Feinberg School of Medicine, Northwestern University, and Blood Bank, Northwestern Memorial Hospital  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9721-5259","authenticated-orcid":false,"given":"Glenn","family":"Ramsey","sequence":"additional","affiliation":[{"name":"Department of Pathology Feinberg School of Medicine, Northwestern University, and Blood Bank, Northwestern Memorial Hospital  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4482-0417","authenticated-orcid":false,"given":"Jay P.","family":"Hudgins","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nationwide Children's Hospital  Columbus Ohio 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