{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:58:24Z","timestamp":1790773104398,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009033","name":"NHS Blood and Transplant","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Clinical guidelines recommend transfusing ABO\u2010identical platelets where possible. However, the short shelf life of platelets (5\u20137\u2009days) and stock availability mean ABO\u2010nonidentical platelet transfusions are sometimes unavoidable. Minor ABO\u2010incompatible platelet transfusions carry a risk of hemolysis due to donor anti\u2010A\/B antibodies, and high\u2010titer (HT)\u2010negative units are preferred to mitigate this risk. To further mitigate this risk, we investigated whether an ABO antibody immunoadsorption column could reduce anti\u2010A\/B titers in group O platelet units while preserving platelet quality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Six group O double dose apheresis platelet units were pooled with HT\u2010positive group O plasma and split into three arms: untreated control, single\u2010pass filtration (Glycosorb ABO A\/B\u2010column), and double\u2010pass using the same column. Anti A and anti B IgM and IgG titers were measured pre\u2010 and post\u2010filtration using a gel card method. Platelet count, platelet volume, coagulation factors, and markers of platelet quality and activation were assessed after filtration and during storage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Anti\u2010A and anti\u2010B titers were decreased from titers of 128 to \u22644 (IgM) and from 512 to \u22648 (IgG) following a single pass through the immunoadsorption column. Reduced efficacy was observed following a second pass through the same column (\u226432 (IgM) and \u2264128 (IgG)). Filtration was not associated with substantial platelet loss, although changes in some metabolic and activation markers were observed during storage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The ABO antibody immunoadsorption column effectively reduced anti\u2010A\/B titers in group O platelet units and may offer a strategy to increase the availability of low\u2010titer platelets for transfusion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70410","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:39:05Z","timestamp":1790681945000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of an\n                    <scp>ABO<\/scp>\n                    antibody immunoadsorption column for reduction of anti\u2010A and anti\u2010B isoagglutinin titers in group O platelet units"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2131-2281","authenticated-orcid":false,"given":"Melanie J.","family":"Robbins","sequence":"first","affiliation":[{"name":"Component Development NHS Blood and Transplant  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Proffitt","sequence":"additional","affiliation":[{"name":"Component Development NHS Blood and Transplant  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Eastwood","sequence":"additional","affiliation":[{"name":"Component Development NHS Blood and Transplant  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5611-554X","authenticated-orcid":false,"given":"Sian","family":"Huish","sequence":"additional","affiliation":[{"name":"Component Development NHS Blood and Transplant  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Nilsson","sequence":"additional","affiliation":[{"name":"GlycoRex Transplantation AB  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6823-8937","authenticated-orcid":false,"given":"Rebecca","family":"Cardigan","sequence":"additional","affiliation":[{"name":"Component Development NHS Blood and Transplant  Cambridge UK"},{"name":"Department of Haematology University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/09537104.2021.1961711"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13129"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14423"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-393165"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12045"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000150"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajts.ajts_145_21"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2012.01.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15138"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2022.100989"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000521217"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2023.103686"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.768458"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13350"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(82)90248-1"},{"key":"e_1_2_9_17_1","unstructured":"Research CfDEa.Statistical approaches to establishing bioequivalence [Monograph on the internet].2001[cited September 28 2026]. Available from:https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/statistical\u2010approaches\u2010establishing\u2010bioequivalence"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13697"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70410","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.70410","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.70410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:12:20Z","timestamp":1790770340000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.70410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":17,"alternative-id":["10.1111\/trf.70410"],"URL":"https:\/\/doi.org\/10.1111\/trf.70410","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"trf.70410"}}