{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:49:48Z","timestamp":1792046988572,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n As cold\u2010stored platelets (CSPs) are being integrated into clinical practice, it is necessary to determine the storage practices that best support platelet quality while remaining operationally feasible. Platelet components are traditionally stored at room temperature, in a single layer, to facilitate gas exchange. The reduced metabolic activity of CSPs may allow for the storage of platelet bags in different configurations, offering improved space efficiency in blood bank refrigerators.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Matched apheresis platelets (\n n<\/jats:italic>\n \u2009=\u20098) in 40% plasma\/60% PAS\u2010E (SSP+) were refrigerated (2\u20136\u00b0C) in one of the following storage configurations: single layer (control), stored upright, or stacked flat. In vitro quality parameters were assessed at defined intervals over storage for 22\u2009days.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Visible aggregates were identified in 75% of components that were stored upright between days 15 and 22, while no aggregates formed in control or stacked components during the storage period. Platelet components stored upright were more activated at maximal expiry, with greater loss of platelet glycoproteins (GPIb\u03b1 and GPVI), and an increase in activation markers (P\u2010selectin, annexin\u2010V, and CD61\n +<\/jats:sup>\n \/annexin\u2010V\n +<\/jats:sup>\n extracellular vesicles). Comparatively, no significant differences in platelet activation markers were observed in stacked platelets at the end of storage compared to controls. No differences in the metabolic or functional capacity of CSPs were observed, regardless of storage configuration.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Platelet components stored upright were more activated and prone to aggregate formation over 22\u2009days of storage. In contrast, stacking is a practical storage option that preserves platelet quality while minimizing space requirements in refrigerators and cool rooms.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.70428","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:31:06Z","timestamp":1792045866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stacking platelet bags: A practical storage configuration for maintaining platelet quality during cold storage"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7801-388X","authenticated-orcid":false,"given":"Janhavi","family":"Mahajan","sequence":"first","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood Alexandria New South Wales Australia"},{"name":"School of Life Sciences University of Technology Sydney Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pearl","family":"Lei","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood Alexandria New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5703-5760","authenticated-orcid":false,"given":"Christopher","family":"Roan","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood Alexandria New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-0643","authenticated-orcid":false,"given":"Matthew P.","family":"Padula","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Technology Sydney Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3674-6934","authenticated-orcid":false,"given":"Denese C.","family":"Marks","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood Alexandria New South Wales Australia"},{"name":"Sydney Medical School University of Sydney Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3303-4437","authenticated-orcid":false,"given":"Lacey","family":"Johnson","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood Alexandria New South Wales Australia"},{"name":"School of Life Sciences University of Technology Sydney Sydney New South Wales Australia"},{"name":"School of Science RMIT University Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Guide to the preparation, use and quality assurance of blood components","author":"Council of Europe","year":"2025"},{"key":"e_1_2_10_3_1","first-page":"172","article-title":"Enhancing uniformity and overall quality of red cell concentrate with anaerobic storage","volume":"15","author":"Yoshida T","year":"2017","journal-title":"Blood Transfus"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/j.1537-2995.2006.00803.x","article-title":"Storage of buffy coat\u2010derived platelets in additive solutions: in vitro effects of storage at 4\u00b0C","volume":"46","author":"Sandgren P","year":"2006","journal-title":"Transfusion"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1111\/trf.13630","article-title":"Refrigeration and cryopreservation of 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