{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:54:52Z","timestamp":1791312892031,"version":"4.3.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T00:00:00Z","timestamp":1412294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports and Culture of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MicroRNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>)\u2010203 is downregulated and acts as an anti\u2010oncomir in melanoma cells. Here, using human and canine melanoma cells, we elucidated the effects of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010203 on cyclic adenosine monophosphate response element binding protein (<jats:styled-content style=\"fixed-case\">CREB<\/jats:styled-content>)\/microphthalmia\u2010associated transcription factor (<jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content>)\/<jats:styled-content style=\"fixed-case\">RAB27a<\/jats:styled-content> pathway, which is known to be important for the development and progression of human melanoma. In this study, we showed that <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010203 directly targeted <jats:italic><jats:styled-content style=\"fixed-case\">CREB1<\/jats:styled-content><\/jats:italic> and regulated its downstream targets, <jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RAB27a<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010203 significantly suppressed the growth of human and canine melanoma cells and inhibited melanosome transport through the suppression of the signalling pathway. In conclusion, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010203 was shown to be a common tumour\u2010suppressive <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content> in human and canine melanoma and thus to play a crucial role in the biological mechanisms of melanoma development.<\/jats:p>","DOI":"10.1111\/vco.12118","type":"journal-article","created":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T07:07:50Z","timestamp":1412320070000},"page":"384-394","source":"Crossref","is-referenced-by-count":30,"title":["Analysis of <scp>microRNA<\/scp>\u2010203 function in <scp>CREB<\/scp>\/<scp>MITF<\/scp>\/<scp>RAB27a<\/scp> pathway: comparison between canine and human melanoma cells"],"prefix":"10.1111","volume":"14","author":[{"given":"S.","family":"Noguchi","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnostic and Therapeutics Joint faculty of Veterinary Medicine, Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kumazaki","sequence":"additional","affiliation":[{"name":"The United Graduate School of Drug Discovery and Medical Information Sciences Gifu University  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Mori","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Oncology Faculty of Applied Biological Sciences, Gifu University  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Baba","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine Joint Faculty of Veterinary Medicine, Yamagushi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Okuda","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine Joint Faculty of Veterinary Medicine, Yamagushi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostic and Therapeutics Joint faculty of Veterinary Medicine, Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Akao","sequence":"additional","affiliation":[{"name":"The United Graduate School of Drug Discovery and Medical Information Sciences Gifu University  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra052166"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-2362-5.00019-0"},{"key":"e_1_2_7_4_1","first-page":"8","article-title":"Vaccination with human tyrosinase DNA induces antibody responses in dogs with advanced melanoma","volume":"6","author":"Liao JC","year":"2006","journal-title":"Cancer Immunity"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12185"},{"key":"e_1_2_7_6_1","first-page":"397","article-title":"New drugs in melanoma: it's a whole new world","volume":"104","author":"Eggermont AM","year":"2011","journal-title":"European Journal of Cancer"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-012-9358-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03664"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800130200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813490754"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22531"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0361224"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1884710"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2005.06.012"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-178186"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.2008.00061.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20080213"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-012-1309-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2012.696102"},{"key":"e_1_2_7_21_1","first-page":"209","article-title":"MicroRNAs in cancer \u2010 from research to therapy","volume":"2010","author":"Cho WC","year":"1805","journal-title":"Biochimica et Biophysica Acta"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.01.008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0264"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2011.00306.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.325027"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.310"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2774"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvs009"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.04.005"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djs373"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701185"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-0823-4"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08782.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01274.x"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvco.12118","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.12118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:57:30Z","timestamp":1695977850000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vco.12118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,3]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/vco.12118"],"URL":"https:\/\/doi.org\/10.1111\/vco.12118","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"value":"1476-5810","type":"print"},{"value":"1476-5829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,3]]}}}