{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:40Z","timestamp":1787015980061,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T00:00:00Z","timestamp":1423440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Liquid\u2010based Cytology (\n                    <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>\n                    ) consists of immediate wet cell fixation with automated slide preparation. We applied\n                    <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>\n                    , cell block (\n                    <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\n                    ) and immunocytochemistry to diagnose canine lymphoma and compare results with conventional cytology. Samples from enlarged lymph nodes of 18 dogs were collected and fixed in preservative solution for automated slide preparation (\n                    <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>\n                    ), CB inclusion and immunophenotyping. Two\n                    <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\n                    techniques were tested: fixed sediment method (\n                    <jats:styled-content style=\"fixed-case\">FSM<\/jats:styled-content>\n                    ) and agar method (\n                    <jats:styled-content style=\"fixed-case\">AM<\/jats:styled-content>\n                    ). Anti\u2010\n                    <jats:styled-content style=\"fixed-case\">CD79a<\/jats:styled-content>\n                    , anti\u2010Pax5, anti\u2010\n                    <jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content>\n                    and anti\u2010Ki67 were used in immunocytochemistry.\n                    <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>\n                    smears showed better nuclear and nucleolar definition, without cell superposition, but presented smaller cell size and worse cytoplasmic definition.\n                    <jats:styled-content style=\"fixed-case\">FSM<\/jats:styled-content>\n                    showed consistent cellular groups and were employed for immunocytochemistry, while\n                    <jats:styled-content style=\"fixed-case\">AM<\/jats:styled-content>\n                    CBs presented sparse groups of lymphocytes, with compromised analysis. Anti\u2010Pax\u20105 allowed B\u2010cell identification, both in reactive and neoplastic lymph nodes. Our preliminary report suggests that\n                    <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">FSM<\/jats:styled-content>\n                    together may be promising tools to improve lymphoma diagnosis through fine\u2010needle aspiration.\n                  <\/jats:p>","DOI":"10.1111\/vco.12137","type":"journal-article","created":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T07:17:23Z","timestamp":1423466243000},"page":"107-116","source":"Crossref","is-referenced-by-count":24,"title":["Liquid\u2010based cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples"],"prefix":"10.1111","volume":"14","author":[{"given":"N. C.C. A.","family":"Fernandes","sequence":"first","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"R\u00e9ssio","sequence":"additional","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. G.","family":"Wasques","sequence":"additional","affiliation":[{"name":"HISTOPET  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Etlinger\u2010Colonelli","sequence":"additional","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lorente","sequence":"additional","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Centro de Patologia Instituto Adolfo Lutz  S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L. Z.","family":"Dagli","sequence":"additional","affiliation":[{"name":"Departamento de Patologia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo  S\u00e3o\u00a0Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.0695.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2014.02.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810379428"},{"key":"e_1_2_7_5_1","first-page":"535","volume-title":"The Art and Science of Cytopathology","author":"DeMay RM","year":"2012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2115"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.3.197"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199801)18:1<24::AID-DC5>3.0.CO;2-U"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2009.00205.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12185"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000296488.85807.b3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(00)00192-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328377"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326413"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22963"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12011"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1955)8:5<948::AID-CNCR2820080515>3.0.CO;2-M"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700404"},{"key":"e_1_2_7_19_1","first-page":"977","article-title":"Managing the canine lymphosarcoma patient in general practice","volume":"48","author":"Rutley M","year":"2007","journal-title":"The Canadian Veterinary Journal"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2011.02.004"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813478210"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063871002200306"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22842"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/G035-P2MM-D1TM-T5QE"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.5795\/jjscc.49.449"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00456.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21352"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000357658"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12078"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3180ca8a49"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.11.019"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12343"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvco.12137","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.12137","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T17:24:41Z","timestamp":1694539481000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vco.12137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,9]]},"references-count":31,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1111\/vco.12137"],"URL":"https:\/\/doi.org\/10.1111\/vco.12137","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"value":"1476-5810","type":"print"},{"value":"1476-5829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,9]]}}}