{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:04Z","timestamp":1787016184394,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T00:00:00Z","timestamp":1453334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cancer is a prevalent cause of mortality in Bernese mountain dogs (\n                    <jats:styled-content style=\"fixed-case\">BMDs<\/jats:styled-content>\n                    ). Circulating\n                    <jats:styled-content style=\"fixed-case\">microRNAs<\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content>\n                    ) are found in blood and have been identified as promising biomarkers in various neoplastic diseases in humans. In the current study, the expression profile of different types of\n                    <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content>\n                    was investigated in healthy\n                    <jats:styled-content style=\"fixed-case\">BMDs<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">BMDs<\/jats:styled-content>\n                    with cancer. Seven healthy and six non\u2010treated\n                    <jats:styled-content style=\"fixed-case\">BMDs<\/jats:styled-content>\n                    with cancer [four with disseminated histiocytic sarcomas (\n                    <jats:styled-content style=\"fixed-case\">DHS<\/jats:styled-content>\n                    )] were enrolled in this study. Clinical evaluations including physical examination, blood analysis, urinalysis and diagnostic imaging were performed on all dogs. Twenty\u2010four different\n                    <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content>\n                    were profiled from\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    isolated from whole blood preserved in\n                    <jats:styled-content style=\"fixed-case\">PAXgene<\/jats:styled-content>\n                    <jats:sup>\u00ae<\/jats:sup>\n                    tubes using quantitative real\u2010time\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>\n                    ). The\n                    <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content>\n                    let\u20107g was significantly down\u2010regulated in dogs with cancer (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.002) and dogs with\n                    <jats:styled-content style=\"fixed-case\">DHS<\/jats:styled-content>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.011) compared with healthy controls. This\n                    <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content>\n                    is a known tumour suppressor and further analyses are warranted to assess its value as a non\u2010invasive biomarker for early detection of different types of cancer in\n                    <jats:styled-content style=\"fixed-case\">BMDs<\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/vco.12196","type":"journal-article","created":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T01:16:11Z","timestamp":1453338971000},"page":"525-533","source":"Crossref","is-referenced-by-count":10,"title":["Circulating let\u20107g is down\u2010regulated in Bernese Mountain dogs with disseminated histiocytic sarcoma and carcinomas\u00a0\u2013\u00a0a prospective study"],"prefix":"10.1111","volume":"15","author":[{"given":"B.","family":"B\u00f8rresen","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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