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Local tumour control, recurrence rate, as well as safety of combined therapy were evaluated. One month after the therapy, no side effects were recorded and good local tumour control was observed with high complete responses rate which even increased during the observation period to 72%. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 gene electrotransfer resulted in 78% of patients with detectable serum <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and\/or <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 levels. In the treated tumours vascular changes as well as minimal T\u2010lymphocytes infiltration was observed. After 1 week, the plasmid <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was not detected intra\u2010 or peritumorally and no horizontal gene transfer was observed. In summary, our study demonstrates high antitumour efficacy of electrochemotherapy combined with <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 electrotransfer, which also prevented recurrences or distant metastases, as well as its safety and feasibility in treatment of canine mast cell tumours.<\/jats:p>","DOI":"10.1111\/vco.12208","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T17:05:27Z","timestamp":1454519127000},"page":"641-654","source":"Crossref","is-referenced-by-count":77,"title":["Efficacy and safety of electrochemotherapy combined with peritumoral IL\u201012 gene electrotransfer of canine mast cell tumours"],"prefix":"10.1111","volume":"15","author":[{"given":"M.","family":"Cemazar","sequence":"first","affiliation":[{"name":"Department of Experimental Oncology Institute of Oncology Ljubljana  Ljubljana Slovenia"},{"name":"Faculty of Health Sciences University of Primorska  Isola Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ambrozic Avgustin","sequence":"additional","affiliation":[{"name":"Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Pavlin","sequence":"additional","affiliation":[{"name":"Veterinary Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Sersa","sequence":"additional","affiliation":[{"name":"Department of Experimental Oncology Institute of Oncology Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Poli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Krhac Levacic","sequence":"additional","affiliation":[{"name":"Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Tesic","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Primorska  Isola Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Lampreht Tratar","sequence":"additional","affiliation":[{"name":"Department of Experimental Oncology Institute of Oncology Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rak","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Primorska  Isola Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Tozon","sequence":"additional","affiliation":[{"name":"Veterinary Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-925X-13-29"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X13507071"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00425.x"},{"key":"e_1_2_7_5_1","first-page":"606","article-title":"Electrochemotherapy for the treatment of recurring aponeurotic fibromatosis in a dog","volume":"54","author":"Spugnini EP","year":"2013","journal-title":"Can Vet J"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.0117.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8019.2010.01370.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00588"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-3141"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.28131"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-008-0204-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-015-1724-2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-199501000-00004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/raon-2015-0005"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/10445490150504666"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.8.22.9734"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10019-012-0044-9"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2003.08.002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1514"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-194-9_24"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523214666141127093654"},{"key":"e_1_2_7_22_1","unstructured":"Hubbard W. 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