{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:06Z","timestamp":1787016006173,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Abstract<\/jats:title>\n \n Cutaneous mast cell tumours (\n MCT<\/jats:styled-content>\n ) are the most common skin tumour in dogs, and to our knowledge, there are no previous studies regarding the global methylation of these tumours.\n DNA<\/jats:styled-content>\n hypomethylation and hypermethylation have been described in several tumours and both mechanisms can lead to carcinogenesis. The purpose of this study was to evaluate the global\n DNA<\/jats:styled-content>\n methylation in canine\n MCT<\/jats:styled-content>\n . A total of 48\n MCT<\/jats:styled-content>\n samples were classified in grades 1, 2 and 3 or high\u2010grade or low\u2010grade. Monoclonal antibodies were used for the immunohistochemical detection of the 5\u2010methylcytosine. The immunostained nuclei were classified in strong, weak or negative pattern, and these were quantified in five distinct microscopic fields (40\u00d7 objective) in each slide. The results showed that global\n DNA<\/jats:styled-content>\n hypomethylation was predominant in grade 3, high\u2010grade, less differentiated\n MCT<\/jats:styled-content>\n . These epigenetic changes in neoplastic mast cells warrant further detailed investigation aiming the establishment of tumour epigenetic therapies.\n <\/jats:p>","DOI":"10.1111\/vco.12241","type":"journal-article","created":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T11:36:26Z","timestamp":1462275386000},"page":"1014-1018","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of the global\n DNA<\/scp>\n methylation in canine mast cell tumour samples by immunostaining of 5\u2010methyl cytosine"],"prefix":"10.1111","volume":"15","author":[{"given":"C. Y.","family":"Morimoto","sequence":"first","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. V.","family":"Tedardi","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. I. M.","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. C.","family":"Kimura","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. S.","family":"Sanches","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. F.","family":"Epiphanio","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"de Francisco Strefezzi","sequence":"additional","affiliation":[{"name":"School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L. Z.","family":"Dagli","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1935(86)90002-3"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00003-2"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098588402100503"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810386469"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.06.036"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2011-0902"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.5.689"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1045"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-011-9286-z"},{"key":"e_1_2_3_11_1","first-page":"1","article-title":"The immunophenotype of mast cells and its utility in the diagnostic work\u2010up of systemic mastocytosis","volume":"97","author":"Teodosio C","year":"2014","journal-title":"J Leukoc Biol"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.61.3.233"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.101683"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2009.136"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12625"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209145"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083558"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083557"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi.09.33"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvco.12241","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvco.12241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.12241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T15:45:46Z","timestamp":1696002346000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vco.12241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,3]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/vco.12241"],"URL":"https:\/\/doi.org\/10.1111\/vco.12241","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"value":"1476-5810","type":"print"},{"value":"1476-5829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,3]]}}}