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MicroRNA (miRNA) dysregulation has been reported in human HCC and shown to have diagnostic and prognostic value; however, there are no data on miRNA expression in canine HCC. The aim of the present study was to investigate differentially expressed miRNAs in canine HCC. Analysis of miRNA expression in canine HCC tissues and cell lines by quantitative reverse transcription PCR showed that miR\u20101, miR\u2010122, let\u20107a, and let\u20107g were downregulated, whereas miR\u201010b and miR\u201021 were upregulated in canine HCC. <jats:italic>MET<\/jats:italic> is one of the target genes of miR\u20101. MET was upregulated in canine HCC at the gene and protein levels, and a significant correlation between the concomitant downregulation of miR\u20101 and upregulation of <jats:italic>MET<\/jats:italic> was observed. Fast\/intermediate\u2010proliferating canine HCC cell lines had higher MET gene and protein expression levels than the slow\u2010proliferating cell line. These findings suggest that miRNAs are differentially expressed in canine HCC, and that the miR\u20101\/MET pathway may be associated with canine HCC cell proliferation.<\/jats:p>","DOI":"10.1111\/vco.12379","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T12:36:35Z","timestamp":1514982995000},"page":"288-296","source":"Crossref","is-referenced-by-count":25,"title":["Aberrant expression of microRNAs and the miR\u20101\/MET pathway in canine hepatocellular carcinoma"],"prefix":"10.1111","volume":"16","author":[{"given":"Y.\u2010C.","family":"Lai","sequence":"first","affiliation":[{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Ushio","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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