{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:57Z","timestamp":1787016177546,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,24]],"date-time":"2019-03-24T00:00:00Z","timestamp":1553385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>\n                    T\u2010cell lymphomas (TCL) are a diverse group of neoplasms with variable diagnostic features, pathophysiologies, therapeutic responses and clinical outcomes. In dogs, TCL includes indolent and aggressive tumours such as T\u2010zone lymphoma (TZL) and peripheral T\u2010cell lymphoma (PTCL), respectively. Delineation of molecular subtypes and investigation into underlying pathophysiologies of aggressive TCLs remains inadequate. We investigate the correlations between flow cytometry and histopathology of 73 cases of nodal TCL. The majority of cases (82.2%) were characterized as CD4\n                    <jats:sup>+<\/jats:sup>\n                    TCL by flow cytometry. Fewer cases were classified as CD8\n                    <jats:sup>+<\/jats:sup>\n                    TCL (6.8%) or CD4\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD8\n                    <jats:sup>\u2212<\/jats:sup>\n                    TCL (11.0%). All cases, regardless of immunophenotype, exhibited conserved histologic features consistent with the WHO classification of PTCL. Histologic subsets of PTCL corresponding to immunophenotypic features were not identified. Neoplastic cell size determined by flow cytometry correlated significantly with mitotic rate. RNA\u2010seq was performed on a subset of CD4\n                    <jats:sup>+<\/jats:sup>\n                    PTCL cases (n\u2009=\u20096) and compared with sorted control CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cells. The gene expression pattern of CD4\n                    <jats:sup>+<\/jats:sup>\n                    PTCL was similar between all cases regardless of breed. PTCL was enriched in pathways representing G\u2010coupled protein receptor signalling, extracellular matrix remodelling and vascular development, immune signalling and mitotic activity. Furthermore, global gene expression changes were consistent with downregulation of PTEN signalling and upregulation of the MTOR\u2010PI3K\u2010ATK axis. In this study, we evaluated the correlations between flow cytometry, histopathology and gene expression within a large cohort of nodal TCLs. We further demonstrate the ability of flow cytometry to identify a subtype of T\u2010cell lymphoma, CD4+ PTCL, with a uniform histomorphology and gene expression profile.\n                  <\/jats:p>","DOI":"10.1111\/vco.12460","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T21:48:15Z","timestamp":1548452895000},"page":"253-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Canine CD4+ T\u2010cell lymphoma identified by flow cytometry exhibits a consistent histomorphology and gene expression profile"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0903-8278","authenticated-orcid":false,"given":"Lauren J.","family":"Harris","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly L.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. J.","family":"Ehrhart","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"},{"name":"Ethos Diagnostic Science Division Ethos Veterinary Health  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia D.","family":"Labadie","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne C.","family":"Avery","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences Colorado State University  Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci3020011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.0133.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12343"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12024"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12304"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2017.07.008"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.05.009"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-4613"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.194449.115"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3330"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-082411-113907"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093765"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-1-92"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810379428"},{"key":"e_1_2_7_16_1","volume-title":"WHO 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