{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:11:04Z","timestamp":1792195864069,"version":"4.4.0"},"reference-count":83,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,2]],"date-time":"2019-06-02T00:00:00Z","timestamp":1559433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D15CA\u2010015"],"award-info":[{"award-number":["D15CA\u2010015"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>Cancer\u2010testis antigens (CTAs) are a category of self proteins aberrantly expressed in diverse malignancies, mostly solid tumours, due to epigenetic de\u2010repression. Normally expressed only in fetal or gametogenic tissues, CTAs are tantalizing immunotherapy targets, since autoimmunity risks appear minimal. Few prevalent CTAs have been identified in human hematologic cancers, and just two in their veterinary counterparts. We sought to discover new CTAs in canine hematologic cancers such as histiocytic sarcoma (HS) and lymphoma to foster immunotherapy development. To accomplish this, the ligandome binding the dog leukocyte antigen (DLA)\u201088*508:01 class I allele overexpressed in an HS line was searched by mass spectrometry to identify possible CTA\u2010derived peptides, which could serve as CD8+ T\u2010cell epitopes. Twenty\u2010two peptides mapped to 5 human CTAs and 12 additional proteins with CTA characteristics. Expression of five promising candidates was then evaluated in tumour and normal tissue by quantitative and end\u2010point RT\u2010PCR. The ortholog of an established CTA, IGF2BP3, had unexpectedly high expression in peripheral blood mononuclear cells (PBMCs). Four other testis\u2010enhanced proteins were also assessed. AKR1E2, SPECC1 and TPX2 were expressed variably in HS and T\u2010cell lymphoma biopsies, but also at high levels in critical tissues, including kidney, brain and marrow, diminishing their utility. A more tissue\u2010restricted candidate, NT5C1B, was detected in T\u2010cell lymphomas, but also at low levels in some normal dog tissues. These results illustrate the feasibility of discovering canine CTAs by a reverse approach, proceeding from identification of MHC class I\u2010presented peptides to a comparative RNA expression survey of tumours and normal tissues.<\/jats:p>","DOI":"10.1111\/vco.12475","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T05:29:35Z","timestamp":1552282175000},"page":"317-328","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cancer\u2010testis antigens in canine histiocytic sarcoma and other malignancies"],"prefix":"10.1111","volume":"17","author":[{"given":"Paige S.","family":"Nemec","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences North Carolina State University, College of Veterinary Medicine  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Kapatos","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences North Carolina State University, College of Veterinary Medicine  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C.","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences North Carolina State University, College of Veterinary Medicine  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devorah M.","family":"Stowe","sequence":"additional","affiliation":[{"name":"Department of Population, Health and Pathobiology North Carolina State University College of Veterinary Medicine  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8146-547X","authenticated-orcid":false,"given":"Paul R.","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences North Carolina State University, College of Veterinary Medicine  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,2]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","first-page":"23","article-title":"Combining RPL27 with OAZ1 or RPL8 as universal normalizers of gene expression in canine endometrial studies","volume":"1","author":"Mair G","year":"2017","journal-title":"Int J Vet Sci Technol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-0116"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4967"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1129003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-281931"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0737"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1100"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-211714"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22991"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature23003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840703"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2725"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.32.2537"},{"key":"e_1_2_9_15_1","unstructured":"ClinicalTrials.gov.Actively\u2010recruiting human clinical ACT trials against the CTA NY\u2010ESO\u20101.2018;https:\/\/clinicaltrials.gov\/ct2\/home. 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