{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:23:21Z","timestamp":1767626601299,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T00:00:00Z","timestamp":1556150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>A cell line named FB\u2010LCH01, derived from a dog diagnosed with Langerhans cell histiocytosis (LCH), was established and characterized. FB\u2010LCH01 had C\u2010shaped nucleoli, characterized by modal chromosome aberrations. The original tumour cells as well as established FB\u2010LCH01 cells were immunopositive for human leukocyte antigen\u2010DR, Iba\u20101 and E\u2010cadherin, and immunonegative for CD163 and CD204, suggesting Langerhans cell origin. Furthermore, the characteristics of FB\u2010LCH01 were compared with those of two canine histiocytic sarcoma cell lines (PWC\u2010HS01 and FCR\u2010HS02) established previously. Expression of E\u2010cadherin was detected only in FB\u2010LCH01, but not in PWC\u2010HS01 and FCR\u2010HS02. All (n\u2009=\u20099) the severe combined immunodeficiency mice inoculated with the FB\u2010LCH01 cells developed subcutaneous tumour masses after 3\u2009weeks. Eight of nine mice also developed metastatic lesions in the lymph nodes (8\/8; 100%), lung (5\/8; 62.5%), stomach (5\/8; 62.5%), heart (4\/8; 50%), pancreas (4\/8; 50%), kidney (3\/8; 37.5%), skin (3\/8; 37.5%) and bone marrow (1\/8; 12.5%). Tumour cells were pleomorphic and round\u2010 to polygonal\u2010shaped with prominent anisocytosis and anisokaryosis. The xenotransplanted tumour cells maintained the immunohistochemical features of the original tumour with persistent E\u2010cadherin expression at injection site and some visceral organs. In conclusion, the established cell line as well as the mice xenotransplant model in this study reflect the nature of canine LCH and may serve as promising models for investigating the patho\u2010tumorigenesis and therapy of the disease.<\/jats:p>","DOI":"10.1111\/vco.12476","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T23:20:39Z","timestamp":1552951239000},"page":"345-353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Establishment of cell line and in vivo mouse model of canine Langerhans cell histiocytosis"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5392-132X","authenticated-orcid":false,"given":"Nguyen V.","family":"Son","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo 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