{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:50Z","timestamp":1787016050303,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T00:00:00Z","timestamp":1585353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"JSPS KAKENHI","award":["JP18K0596408"],"award-info":[{"award-number":["JP18K0596408"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Canine malignant melanoma is a common cancer with a high mortality rate and is a clinically important disease. DNA methylation has been considered to be a potential tumorigenic mechanism through aberrant DNA methylation at promoter region which represses gene transcription. Global hypomethylation could also facilitate chromosome instability. There are few reports regarding DNA methylation in canine malignant melanoma; therefore, the purpose of this study was to examine DNA methylation status of long interspersed nucleotide element\u20101 (LINE\u20101) to be a surrogate marker of genome\u2010wide methylation changes in this disease. We measured levels of DNA methylation of two adjacent cytosine\u2010guanine sites on CpG island (CGI) at the putative promoter of canine LINE\u20101 sequence by bisulphite\u2010pyrosequencing in 41 canine melanoma patient samples as well as six cell lines compared with normal mucosae. The survival rates were obtained from owners or medical records. We found DNA methylation levels of LINE\u20101 in normal mucosae were methylated. Interestingly, both melanoma cell lines and clinical melanoma samples showed remarkable hypomethylation. In addition, patients with lower LINE\u20101 methylation showed worse prognosis than those with higher LINE\u20101 methylation, though the difference did not reach statistical significance (\n                    <jats:italic>P<\/jats:italic>\n                    =\u2009.09). Here, we demonstrate that hypomethylation of LINE\u20101 is an epigenetically aberrant feature in canine melanoma with possible prognostic value.\n                  <\/jats:p>","DOI":"10.1111\/vco.12591","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T17:47:13Z","timestamp":1584553633000},"page":"854-860","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Long interspersed nucleotide element\u20101 hypomethylation in canine malignant mucosal melanoma"],"prefix":"10.1111","volume":"18","author":[{"given":"Teita","family":"Ishizaki","sequence":"first","affiliation":[{"name":"Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3241-7869","authenticated-orcid":false,"given":"Jumpei","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Meagawa","sequence":"additional","affiliation":[{"name":"Department of Pediatrics MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomu","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2987-0793","authenticated-orcid":false,"given":"Keisuke","family":"Aoshima","sequence":"additional","affiliation":[{"name":"Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuyoshi","family":"Takiguchi","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine Hokkaido University  Hokkaido 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