{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:51:44Z","timestamp":1791935504425,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Canine osteosarcoma (OSA) is an aggressive bone tumour in dogs. Standard\u2010of\u2010care treatment typically results in relatively short survival times; thus, alternative treatments are needed to confer a survival advantage. It has been shown that OSA is an immunogenic tumour, suggesting that immune modulation may result in superior outcomes. A cryopreserved, <jats:italic>Listeria<\/jats:italic>\u2010based OSA vaccine was recently developed and an initial study in dogs reported prolonged survival for patients receiving the vaccine in conjunction with standard\u2010of\u2010care. The goal of the current observational study was to report on the safety of the lyophilized formulation of this vaccine (the canine OSA vaccine, live <jats:italic>Listeria<\/jats:italic> vector [COV\u2010LLV]) in a group of dogs previously diagnosed with OSA. Forty\u2010nine (49) dogs received the COV\u2010LLV and were included for analysis. Adverse events (AEs) noted during and after vaccinations were recorded. The AEs observed were typically mild and self\u2010limiting, with nausea, lethargy and fever being most common. Four dogs (8%) cultured positive for <jats:italic>Listeria<\/jats:italic> (three infections including an amputation site abscess, septic stifle joint and bacterial cystitis; and one dog whose lungs cultured <jats:italic>Listeria<\/jats:italic>\u2010positive on necropsy within 24\u2009hours of COV\u2010LLV administration). These cases join the previously reported <jats:italic>Listeria<\/jats:italic>\u2010positive thoracic abscess that developed in a canine following use of COV\u2010LLV. Although uncommon, it is important to realize this clinically significant AE is possible in patients treated with live therapeutic <jats:italic>Listeria<\/jats:italic> vaccines. As <jats:italic>Listeria<\/jats:italic> is zoonotic, caution is required not only for the patient receiving the vaccine, but also for the health care workers and family caring for the patient.<\/jats:p>","DOI":"10.1111\/vco.12642","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T03:48:36Z","timestamp":1596167316000},"page":"92-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Safety evaluation of the canine osteosarcoma vaccine, live <i>Listeria<\/i> vector"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-0614","authenticated-orcid":false,"given":"Margaret L.","family":"Musser","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Veterinary Clinical Sciences Iowa State University  Ames Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika P.","family":"Berger","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Veterinary Clinical Sciences Iowa State University  Ames Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea D.","family":"Tripp","sequence":"additional","affiliation":[{"name":"Oncology Service Bridge Animal Referral Center  Edmonds Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig A.","family":"Clifford","sequence":"additional","affiliation":[{"name":"Oncology Service Hope Veterinary Specialists  Malvern Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Bergman","sequence":"additional","affiliation":[{"name":"VCA Katonah Bedford Veterinary Center, Clinical Studies and Oncology Service  Bedford Hills New York USA"},{"name":"Adjunct Faculty Member Memorial Sloan\u2010Kettering Cancer Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad M.","family":"Johannes","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Veterinary Clinical Sciences Iowa State University  Ames Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12603"},{"key":"e_1_2_8_3_1","volume-title":"Withrow and MacEwen's Small Animal Clinical Oncology","author":"Vail D","year":"2019"},{"issue":"938","key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1093\/postgradmedj\/79.938.672","article-title":"Dr William Coley and tumour regression: a place in history or in the future","volume":"79","author":"Hoption Cann SA","year":"2003","journal-title":"Postgrad Med J"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1245\/ASO.2005.01.011"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2015.528"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532-950X.2006.00185.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2964(74)90133-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/81.12.935"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.937"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.149"},{"issue":"7","key":"e_1_2_8_12_1","first-page":"1868","article-title":"Adjuvant treatment of canine osteosarcoma with the human cytotoxic T\u2010cell line TALL\u2010104","volume":"5","author":"Visonneau S","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0088"},{"key":"e_1_2_8_14_1","unstructured":"Aratana Therapeutics Granted Conditional License for a Canine Osteosarcoma Therapeutic [press release]. Leawood KS: Aratana Therapeutics Inc.2017."},{"key":"e_1_2_8_15_1","unstructured":"Aratana Therapeutics. Canine osteosarcoma vaccine live Listeria vector package In. 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