{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T09:47:16Z","timestamp":1786787236088,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mast cell tumours (MCT) have been documented in numerous species and mutations within the <jats:italic>KIT proto\u2010oncogene<\/jats:italic> are implicated in the neoplastic biology of mast cells in humans, dogs and cats. This study determined high <jats:italic>KIT<\/jats:italic> gene nucleotide and Kit amino acid sequence homology between several species known to suffer mast cell neoplasia and especially high sequence conservation between the cheetah (<jats:italic>Acinonyx jubatus<\/jats:italic>) and domestic cat (<jats:italic>Felis catus<\/jats:italic>) <jats:italic>KIT<\/jats:italic> sequences. As a result, we hypothesised that <jats:italic>KIT<\/jats:italic> mutations would exist in the neoplastic DNA of four cheetahs diagnosed with MCT from a recent case series. PCR and Sanger sequencing identified conservative exon 6 <jats:italic>KIT<\/jats:italic> mutations in two of the four cheetahs. The mutations were different between the two cheetahs. Only wild\u2010type DNA in exons 6, 8, 9 and 11 of <jats:italic>KIT<\/jats:italic> was observed in the MCTs of the remaining two cheetahs. Twenty cutaneous MCTs from domestic cats were collected for <jats:italic>KIT<\/jats:italic> mutation comparison. Twelve tumours possessed a mutation within <jats:italic>KIT<\/jats:italic> exons 6, 8 or 9 (60%, 95% CI 38.5%\u201081.5%). No mutations were detected in exon 11. There was no significant association between domestic feline MCT <jats:italic>KIT<\/jats:italic> mutation status and tumour histological grade (traditional schematic, <jats:italic>P<\/jats:italic>\u00a0=\u2009.934; Sabattini 2\u2010tier schematic, <jats:italic>P<\/jats:italic>\u00a0=\u2009.762) or mitotic index (<jats:italic>P<\/jats:italic>\u00a0=\u2009.750). <jats:italic>KIT<\/jats:italic> mRNA and Kit protein sequences are conserved across species but the role of <jats:italic>KIT<\/jats:italic> in feline MCT pathogenesis is not completely understood.<\/jats:p>","DOI":"10.1111\/vco.12683","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T04:31:10Z","timestamp":1611808270000},"page":"381-392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp><i>KIT<\/i><\/scp> mutations in mast cell tumours from cheetahs (<scp><i>Acinonyx jubatus<\/i><\/scp>) and domestic cats (<scp><i>Felis catus<\/i><\/scp>)"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2732-5369","authenticated-orcid":false,"given":"Vanessa S.","family":"Tamlin","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences The University of Adelaide  Roseworthy Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia D. 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