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In the present study, novel cell lines and xenograft mouse models of systemic histiocytosis (SyH) and disseminated HS were established, and examined together with cell lines previously established from localized HS and Langerhans cell histiocytosis (LCH). The chromosomal numbers of the SyH and HS cell lines were abnormal, and their population doubling time and morphological features were comparable. Immunophenotypically, SyH and HS cells were CD204+\/E\u2010cadherin+ in vitro and in vivo, like their original tissues. Western blot analysis for E\u2010cadherin revealed an immunopositive band of full\u2010length E\u2010cadherin (120\u2009kDa) in cultured cells of localized HS and LCH but not in disseminated HS and SyH; expression level was weaker in localized HS than in LCH. An immunopositive band of fragmented E\u2010cadherin (45\u2009kDa) was detected in cell lines of disseminated HS and SyH. These results suggest that cultured SyH cells have features that are similar to disseminated HS, including chromosomal aberration, high proliferation activity, weak cell adhesion, and expression of fragmented E\u2010cadherin. Fragmentation of the E\u2010cadherin cell adhesion molecule may be associated with the loss of cell adhesion and increased abilities of invasion and migration of neoplastic cells. The established cell lines and xenograft mouse models will aid in understanding the pathogenesis and developing novel treatments of canine histiocytic proliferative disorders.<\/jats:p>","DOI":"10.1111\/vco.12792","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T09:07:10Z","timestamp":1639559230000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and characterisation of cell lines and xenograft mouse models of canine systemic histiocytosis and disseminated histiocytic sarcoma"],"prefix":"10.1111","volume":"20","author":[{"given":"Miyuki","family":"Hirabayashi","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pathology Graduate School of Agricultural and Life Sciences, The University of Tokyo  Tokyo Japan"},{"name":"Saitama Animal Medical Center  Saitama 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