{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:02Z","timestamp":1747188422361,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diffuse large B\u2010cell lymphoma (DLBCL) is frequently treated with chemotherapy incorporating cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP), which induces remission in 80% to 95% of cases. However, not all dogs derive meaningful benefit from CHOP, and prognostic factors for dogs with DLBCL are poorly defined. Serum thymidine kinase 1 (TK1) activity, a marker of tumour cell proliferation, has shown promising initial results as a prognostic biomarker in dogs with multicentric lymphomas. The purpose of this study was to determine if baseline serum TK1 activity is associated with clinical outcome in dogs with CHOP\u2010treated DLBCL. Baseline serum TK1 activity was measured in banked sera from 98 dogs with CHOP\u2010treated DLBCL using a commercially available ELISA kit. Data on other potential prognostic factors were abstracted retrospectively from electronic medical records. Multivariable statistical methods were used to identify associations between TK1 and other potential prognostic factors with progression\u2010free survival (PFS) and attainment of complete remission. TK1 activity at baseline was not associated with PFS (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.299) or attainment of complete remission (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.910) following CHOP chemotherapy. Of the other prognostic factors analysed, only purebred (vs. mixed breed) status (HR 8.81, 95% CI 1.68\u201346.30, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.010), attainment of complete (vs. partial) remission (HR 0.09, 95% CI 0.02\u20130.49, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.006), and baseline serum C\u2010reactive protein concentration (HR 1.19, 95% CI 1.07\u20131.32, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.001) were independently associated with PFS. Based on these findings, baseline serum TK1 activity does not appear to be a useful prognostic biomarker in dogs with CHOP\u2010treated DLBCL.<\/jats:p>","DOI":"10.1111\/vco.12876","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T12:28:19Z","timestamp":1674044899000},"page":"200-207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serum thymidine kinase 1 activity as a prognostic biomarker in dogs with chemotherapy\u2010treated diffuse large B\u2010cell lymphoma"],"prefix":"10.1111","volume":"21","author":[{"given":"Bushra","family":"Zaidi","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhijit","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"Ramos\u2010Vara","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology College of Veterinary, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepika","family":"Dhawan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Ruple","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology College of Veterinary, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6127-4679","authenticated-orcid":false,"given":"Michael O.","family":"Childress","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine, Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci3020011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12364"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.07.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12263"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12378"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12453"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.1993.8.189"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15513"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2217\/bmm-2018-0157"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2004.tb02608.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2013.779690"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137871"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810379428"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02411.x"},{"key":"e_1_2_9_16_1","unstructured":"BilbroughG.E. 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