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In this study, AIM expression in the tumour region and serum AIM levels in dogs with HS was assessed. Additionally, the effects of AIM overexpression on HS cell viability were investigated using a HS cell line that was selected from five validated HS cell lines. Immunohistochemistry showed that AIM expression was observed in the cytoplasm of the HS cells. CD36, a candidate AIM receptor, was also observed on the cell membrane of HS cells. When the serum AIM level was detected in 36 dogs with HS and 10\u2009healthy dogs via western blot analysis, the AIM levels in the HS dogs were significantly higher than those in the controls. <jats:italic>AIM<\/jats:italic> mRNA expression in the 5 HS cell lines varied but was higher than that in the other tumour\u2010derived lines. Among the five HS cell lines, DH82 originally had lower <jats:italic>AIM<\/jats:italic> and the highest <jats:italic>CD36<\/jats:italic> expression. When AIM was overexpressed in DH82, therein cell growth speed and invasion, apoptosis inhibition and phagocytic activity were strongly upregulated. These data suggest that elevated intra\u2010tumour expression of AIM could induce the progression of HS cells in dogs. Moreover, elevated serum AIM levels in dogs with HS could serve as a biomarker of HS.<\/jats:p>","DOI":"10.1111\/vco.12897","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T01:23:48Z","timestamp":1682299428000},"page":"391-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serum level of apoptosis inhibitor of macrophage in dogs with histiocytic sarcoma and its association with the disease"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5781-9485","authenticated-orcid":false,"given":"Mona","family":"Uchida","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Matsumiya","sequence":"additional","affiliation":[{"name":"Department of 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