{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:58:41Z","timestamp":1790845121080,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enumeration of circulating tumour cells (CTC) has shown promise for prognostication and guidance of therapeutic decisions in human cancers. The objective of this study was to enumerate CTC over time in dogs with naturally occurring osteosarcoma (OSA), and to determine correlation with patient outcome. Twenty\u2010six dogs with OSA and no evidence of metastatic disease at the time of amputation were enrolled. Dogs were assessed for lung metastases and CTC prior to and following amputation, and at each chemotherapy visit. Twenty\u2010one dogs completed the study. Nineteen dogs were euthanized and two were alive and free of metastases. Overall survival time ranged from 88 to 1058\u2009days (median survival time (MST) 374\u2009days). Increased serum alkaline phosphatase activity, advanced age, and higher body weight were significantly associated with lower MST. Dogs with OSA had a mean of 356 (0 to 4443) CTC\/10<jats:sup>6<\/jats:sup>leukocytes. In 12 of 15 dogs that developed radiographic evidence of metastasis, a pre\u2010metastatic CTC spike was retrospectively detectable on average 36.5 (1\u2013100\u2009days) days prior to metastasis and was associated with significantly shorter MST (301\u2009\u00b1\u200964 vs. 626\u2009\u00b1\u200955\u2009days;<jats:italic>p<\/jats:italic>\u2009=\u2009.0107). In a multivariable analysis, dogs with a CTC spike were 10\u00d7 more likely to die compared with those without. These results suggest that a spike in CTC frequency precedes detection of metastasis in dogs with OSA and is associated with shorter survival. More frequent enumeration of CTC in a larger cohort of dogs with OSA may be warranted.<\/jats:p>","DOI":"10.1111\/vco.12918","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T03:20:16Z","timestamp":1687231216000},"page":"541-550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Quantification of circulating tumour cells over time in dogs with appendicular osteosarcoma"],"prefix":"10.1111","volume":"21","author":[{"given":"Tanya F.","family":"Wright","sequence":"first","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College, University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9646-0815","authenticated-orcid":false,"given":"Brigitte A.","family":"Brisson","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College, 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