{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:48Z","timestamp":1787016048210,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T00:00:00Z","timestamp":1695254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001291","name":"Petco Love","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001291","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Canine hemangiosarcoma (HSA) is an aggressive cancer of endothelial cells with short survival times. Understanding the genomic landscape of HSA may aid in developing therapeutic strategies for dogs and may also inform therapies for the rare and aggressive human cancer angiosarcoma. The objectives of this study were to build a framework for leveraging real\u2010world genomic and clinical data that could provide the foundation for precision medicine in veterinary oncology,\u00a0and\u00a0to determine the relationships between genomic and clinical features in canine splenic HSA. One hundred and nine dogs with primary splenic HSA treated by splenectomy that had tumour sequencing via the FidoCure\u00ae Precision Medicine Platform targeted sequencing panel were enrolled. Patient signalment, weight, metastasis at diagnosis and overall survival time were retrospectively evaluated. The incidence of genomic alterations in individual genes and their relationship to patient variables including outcome were assessed. Somatic mutations in\n                    <jats:italic>TP53<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944),\n                    <jats:italic>NRAS<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920) and\n                    <jats:italic>PIK3CA<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200919) were most common. Survival was associated with presence of metastases at diagnosis and germline variants in\n                    <jats:italic>SETD2<\/jats:italic>\n                    and\n                    <jats:italic>NOTCH1<\/jats:italic>\n                    . Age at diagnosis was associated with somatic\n                    <jats:italic>NRAS<\/jats:italic>\n                    mutations and breed.\n                    <jats:italic>TP53<\/jats:italic>\n                    and\n                    <jats:italic>PIK3CA<\/jats:italic>\n                    somatic mutations were found in larger dogs, while germline\n                    <jats:italic>SETD2<\/jats:italic>\n                    variants were found in smaller dogs. We identified both somatic mutations and germline variants associated with clinical variables including age, breed and overall survival. These genetic changes may be useful prognostic factors and provide insight into the genomic landscape of hemangiosarcoma.\n                  <\/jats:p>","DOI":"10.1111\/vco.12925","type":"journal-article","created":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T20:53:26Z","timestamp":1695329606000},"page":"623-633","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of genomic alterations with clinical impact in canine splenic hemangiosarcoma"],"prefix":"10.1111","volume":"21","author":[{"given":"Timothy","family":"Estabrooks","sequence":"first","affiliation":[{"name":"Cummings School of Veterinary Medicine Tufts University  North Grafton Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Gurinovich","sequence":"additional","affiliation":[{"name":"Tufts Medical Center  Boston Massachusetts 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