{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:39:19Z","timestamp":1767339559594,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H02366","20K21375"],"award-info":[{"award-number":["21H02366","20K21375"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>miR\u2010301a is one of numerous dysregulated microRNAs (miRNAs) in canine oral melanoma (COM), one of which is miR\u2010301a (upregulated). Its biological role has been described in various human cancer types, including malignant melanoma, but not in COM. Accordingly, in this study, we investigated miR\u2010301a expression in COM in greater detail to ascertain whether it could serve as a diagnostic biomarker, elucidate its functional roles in this cancer, and predict the possible pathways by which it exerts its effects. Relative expression of miR\u2010301a was investigated in clinical oral tissue and plasma samples and COM cell (KMeC and LMeC) lines using qRT\u2010PCR. Knockdown of miR\u2010301a was also validated for KMeC and LMeC cells using qRT\u2010PCR. We performed CCK\u20108 assays to assess cell proliferation, monolayer wound\u2010healing, and transwell migration assays to assess cell migration, a colony\u2010formation assay to assess clonogenicity, a TUNEL assay and flow cytometry to assess apoptosis\u2010related effects, and gene enrichment analyses to predict possible related pathways. miR\u2010301a was markedly upregulated in COM oral tissue and plasma clinically, suggesting its potential as a diagnostic biomarker for COM diagnosis. In vitro assays demonstrated that miR\u2010301 significantly inhibited apoptosis in COM cells while promoting cell migration, proliferation, and clonogenicity. We also predicted that miR\u2010301 exerts cancer\u2010promoting effects through the Wnt signalling pathway for COM. Our findings suggest that miR\u2010301a is a COM oncomiR that regulates several oncogenic phenotypes with the potential to be a diagnostic biomarker.<\/jats:p>","DOI":"10.1111\/vco.12954","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T07:18:48Z","timestamp":1703661528000},"page":"78-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Elevated expression of <scp>miR<\/scp>\u2010301a and its functional roles in canine oral melanoma"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8666-972X","authenticated-orcid":false,"given":"MD Nazmul","family":"Hasan","sequence":"first","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"},{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1965-9836","authenticated-orcid":false,"given":"Md. Mahfuzur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Human Oncology University of Wisconsin School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Al Asmaul","family":"Husna","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3432-7696","authenticated-orcid":false,"given":"Mohammad","family":"Arif","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4127-3879","authenticated-orcid":false,"given":"Tomoko","family":"Iwanaga","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Tsukiyama\u2010Kohara","sequence":"additional","affiliation":[{"name":"Transboundary Animal Diseases Centre, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indre","family":"Jasineviciute","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, Veterinary Faculty Lithuanian University of Health Sciences  Kaunas Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiki","family":"Kato","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4625-5231","authenticated-orcid":false,"given":"Naoki","family":"Miura","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"},{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000452540"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985821996658"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2011.00306.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctsap.2007.03.004"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.245.11.1266"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s10038-021-00938-6"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-022-00948-2"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgz121"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.109995"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2020.103196"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010201"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20194832"},{"issue":"1","key":"e_1_2_11_14_1","first-page":"1080","article-title":"Identification of dysregulated microRNAs in canine malignant melanoma","volume":"17","author":"Ushio N","year":"2019","journal-title":"Oncol Lett"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S253533"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.01.034"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1024-0"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20680-y"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369690"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24200"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2020.5072"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-012-2099-2"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omto.2021.03.007"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2020.5145"},{"issue":"1","key":"e_1_2_11_25_1","first-page":"599","article-title":"MicroRNA\u2010301a targets WNT1 to suppress cell proliferation and migration and enhance radiosensitivity in esophageal cancer cells","volume":"41","author":"Su H","year":"2019","journal-title":"Oncol Rep"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2022.146237"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12558"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.66.1437"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.13000"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.339"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010770"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000513070"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.153897"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvaa079"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504018X15166193231711"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20574"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.23374"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S102862"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2019.7391"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.07.001"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.12954","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T06:33:19Z","timestamp":1707719599000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vco.12954"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,26]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/vco.12954"],"URL":"https:\/\/doi.org\/10.1111\/vco.12954","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"type":"print","value":"1476-5810"},{"type":"electronic","value":"1476-5829"}],"subject":[],"published":{"date-parts":[[2023,12,26]]},"assertion":[{"value":"2023-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}