{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T16:16:01Z","timestamp":1789920961529,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18K14586"],"award-info":[{"award-number":["JP18K14586"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Canine lymphoma is a disease with high morbidity and poor long\u2010term prognosis, despite a high response rate to chemotherapy. In this study, we focused on liquid biopsy, in which small amounts of substances from body fluids were analysed, to determine whether cell\u2010free DNA (cfDNA) in the plasma can be used as a biomarker for lymphoma in dogs. We found that 23 patients with lymphoma had significantly higher cfDNA concentrations than the 12 healthy dogs (median 2360\u2009ng\/mL versus 299\u2009ng\/mL,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001). Polymerase chain reaction for antigen receptor rearrangement (PARR) was also employed using cfDNA from the lymphoma group to investigate whether cfDNA could be used for the detection of genetic clonality of lymphomas, as well as the genomic DNA (gDNA) extracted from an original lesion in each case. The correlation of the PARR results between cfDNA and gDNA was observed in 100% of B\u2010cell lymphomas (10\/10), 77.8% of T\u2010cell lymphomas (7\/9), and 100% of other types of lymphomas (4\/4), respectively. These results indicate that plasma cfDNA levels are increasing in canine lymphoma patients, that cfDNA concentration can be a novel diagnostic tool, and that it can be used as a diagnostic tool for PARR.\n                  <\/jats:p>","DOI":"10.1111\/vco.12961","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T23:15:26Z","timestamp":1706570126000},"page":"303-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Plasma cell\u2010free\n                    <scp>DNA<\/scp>\n                    in canine lymphoma patients as a novel material for genotyping"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6792-3980","authenticated-orcid":false,"given":"Satoshi","family":"Kambayashi","sequence":"first","affiliation":[{"name":"Department of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"},{"name":"Laboratory of Veterinary Internal Medicine Joint Graduate School of Veterinary Medicine, Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanae","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomofumi","family":"Tone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6157-2801","authenticated-orcid":false,"given":"Kenji","family":"Baba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"},{"name":"Laboratory of Veterinary Internal Medicine Joint Graduate School of Veterinary Medicine, Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Okuda","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"},{"name":"Laboratory of Veterinary Internal Medicine Joint Graduate School of Veterinary Medicine, Yamaguchi University  Yamaguchi 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