{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T03:40:23Z","timestamp":1723520423143},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/01004\u20103;2017\/22374\u20100"],"award-info":[{"award-number":["2020\/01004\u20103;2017\/22374\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liquid biopsy for circulating tumour cell (CTC) detection is generally unexplored in veterinary medicine. Dogs with highly aggressive and heterogeneous tumours, such as oral malignant melanoma (OMM), could benefit from studies involving size\u2010based isolation methods for CTCs, as they do not depend on specific antibodies. This pilot study aimed to detect CTCs from canine OMM using Isolation by Size of Epithelial Tumor Cells (ISET), a microfiltration methodology, followed by immunocytochemistry (ICC) with Melan\u2010A, PNL2, and S100 antibodies. Ten canine patients diagnosed by histopathology and confirmed as OMM by immunohistochemistry were enrolled, their prognostic data was assessed, and blood samples were collected for CTC analysis. Results have shown the detection of intact cells in 9\/10 patients. ICC has shown 3\/9 Melan\u2010A\u2010positive, 3\/9 PNL2\u2010positive, and 8\/9 S100\u2010positive patients, confirming the importance of opting for a multimarker assay. A significant number of negative\u2010stained CTCs were found, suggesting their high heterogeneity in circulation. Microemboli stained with either PNL2 or S100 were found in a patient with a high isolated cell count and advanced clinical stage. Preliminary statistical analysis shows a significant difference in CTC count between patients with and without lymph node metastasis (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), which may correlate with tumour metastatic potential. However, we recommend further studies with more extensive sampling to confirm this result. This pilot study is the first report of intact CTC detection in canine OMM and the first application of ISET in veterinary medicine, opening new possibilities for liquid biopsy studies in canine OMM and other tumours.<\/jats:p>","DOI":"10.1111\/vco.12982","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T19:56:45Z","timestamp":1717531005000},"page":"388-397","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pilot study to evaluate isolation by size of circulating tumour cells in canine oral melanoma"],"prefix":"10.1111","volume":"22","author":[{"given":"Victor","family":"Nowosh","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexcia Camila","family":"Braun","sequence":"additional","affiliation":[{"name":"Secretaria de Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o do Complexo Econ\u00f4mico\u2010Industrial da Sa\u00fade, Coordena\u00e7\u00e3o Geral de Pesquisa Cl\u00ednica Minist\u00e9rio da Sa\u00fade  Brasilia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Paula Carreta","family":"Ruano","sequence":"additional","affiliation":[{"name":"International Research Center A.C. 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