{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T16:16:06Z","timestamp":1789920966493,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K05952"],"award-info":[{"award-number":["JP21K05952"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Only a limited number of tumour biomarkers are currently available in veterinary medicine, particularly in cats. Cell\u2010free DNA (cfDNA) is an extracellular DNA fragment released upon cell death and is considered a minimally invasive biomarker for the diagnosis and monitoring of various human malignancies. This study aimed to clarify the utility of circulating cfDNA as a liquid biopsy for various feline tumours. Plasma samples were collected from 44 cats with various tumours, 24 cats with other diseases and 10 healthy controls. A follow\u2010up study was conducted in three tumour\u2010bearing patients. All cfDNA concentrations were quantified via real\u2010time polymerase chain reaction (PCR), which provided short and long fragments of a newly identified feline\n                    <jats:italic>LINE\u20101<\/jats:italic>\n                    gene. We found that cfDNA levels were significantly higher in cats with various tumours than in those with other diseases or healthy controls. The cfDNA concentration was not correlated with serum amyloid A (SAA) levels. Cats with tumours exhibited elevated cfDNA levels that predicted tumour\u2010bearing with a sensitivity and specificity of 50.5% and 91.2%, respectively (AUC 0.736;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). In lymphoma cases, cats with high cfDNA levels had significantly shorter survival times than those with low cfDNA levels (median: 33\u2009days vs. 178\u2009days;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003). In addition, the cfDNA levels of the three patients correlated with clinical status during follow\u2010up. Collectively, these findings indicate the potential of cfDNA as a useful biomarker for the diagnosis, therapeutic monitoring and prognostic assessment of tumours in cats.\n                  <\/jats:p>","DOI":"10.1111\/vco.13013","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T10:13:30Z","timestamp":1728555210000},"page":"592-601","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Clinical Utility of Circulating Cell\u2010Free\n                    <scp>DNA<\/scp>\n                    as a Liquid Biopsy in Cats With Various Tumours"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1645-4413","authenticated-orcid":false,"given":"Michihito","family":"Tagawa","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"},{"name":"Veterinary Medical Center Obihiro University of Agriculture and Veterinary Medicine  Obihiro Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hotaka","family":"Hiroi","sequence":"additional","affiliation":[{"name":"Veterinary Medical Center Obihiro University of Agriculture and Veterinary Medicine  Obihiro Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuki","family":"Nakano","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riyo","family":"Morishita","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Sakai","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-5-39"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00046339"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.22-0456"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16616"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CS00370E"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12848"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1448.011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2006.11.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2019.05.002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9AN00935C"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169454"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14808"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vec.12592"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13182987"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0232365"},{"key":"e_1_2_8_17_1","first-page":"1","article-title":"Clinical 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Quantitative Analysis of the Potential Biomarkers of Non\u2010Small Cell Lung Cancer by Circulating Cell\u2010Free DNA","volume":"16","author":"Wei L.","year":"2018","journal-title":"Oncology Letters"}],"container-title":["Veterinary and Comparative 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