{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:56Z","timestamp":1787016236489,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Canine multicentric lymphoma (CML) is one of the most common malignancies in dogs. Although breed risk is important, environmental factors such as herbicides have also been implicated. The objective of this study was to determine whether genotoxic exposures to the herbicides 2,4\u2010D and glyphosate are associated with CML, using dogs from the Golden Retriever Lifetime Study cohort. We measured urinary concentrations of glyphosate and 2,4\u2010D in golden retrievers with CML and matched unaffected controls at two time points: at the time of diagnosis and 1\u2009year prior to diagnosis. To assess the genotoxic potential of herbicide exposures, we used reverse dosimetry from urinary concentrations to estimate plasma concentrations. We then assessed the genotoxicity of these herbicide concentrations towards healthy canine peripheral blood mononuclear cells (PBMC's) in\u00a0vitro using the CometChip assay, with and without canine liver microsomes. All dogs had detectable urinary exposures to 2,4\u2010D (7.3\u201342.9\u2009ng\/mg creat) and glyphosate (0.4\u201380.7\u2009ng\/mg creat), with no differences between cases and controls at either time point. Both 2,4\u2010D and glyphosate were genotoxic to canine PBMCs at concentrations of 0.10 \u03bcM and higher, with no consistent effects of canine liver microsomes on herbicide genotoxicity. No dogs reached estimated genotoxic plasma concentrations for glyphosate, but 4 of 30 golden retrievers with CML (13.3%) and 2 of 30 control dogs (6.7%) reached estimated genotoxic 2,4\u2010D exposures (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.67).\n                  <\/jats:p>","DOI":"10.1111\/vco.13051","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T05:36:03Z","timestamp":1742708163000},"page":"246-256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genotoxic Herbicide Exposures in Golden Retrievers With and Without Multicentric Lymphoma"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1886-2082","authenticated-orcid":false,"given":"Ashleigh N.","family":"Tindle","sequence":"first","affiliation":[{"name":"Department of Medical Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren M.","family":"Krueger","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenna","family":"Swafford","sequence":"additional","affiliation":[{"name":"Scientific Programs Department Morris Animal Foundation  Denver Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Mani","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Danielson","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Labadie","sequence":"additional","affiliation":[{"name":"Scientific Programs Department Morris Animal Foundation  Denver Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8585-7350","authenticated-orcid":false,"given":"Lauren A.","family":"Trepanier","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2016.1152633"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci3020011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1994.9694451"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939\u20101676.2008.0255.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1753\u20106561\u20107\u2010s2\u2010k13"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/83.17.1226"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/enrs.1995.1056"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.13075"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajim.4700180310"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.60.9.e11"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23589"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2011.12.003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/83.17.1226"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2007.05.003"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20569"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2021.639143"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2018.07.035"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0269425"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2014.0230"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/cts.2020.548"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.07.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.12.454"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000119r"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00003-014-0927-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010019\u201007087\u20106"},{"key":"e_1_2_9_27_1","article-title":"The Ins and Outs of Polyuria and Polydipsia","author":"Osborne C. 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