{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T08:28:02Z","timestamp":1749630482252,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Canine transmissible venereal tumour (CTVT) is a common diagnosis in many low\u2010income countries, and managing its chemoresistant cases can be challenging within the conventional vincristine sulfate chemotherapy protocol. Also, predictive markers of chemoresistance for this tumour remain unidentified. This study aimed to evaluate CTVT cytological characteristics to develop a predictive cytological scoring system for vincristine sulfate resistance. For this purpose, 40 cases were retrospectively analysed based on their clinical aspects and response to vincristine chemotherapy. First, cytological preparations underwent a double\u2010blind assessment to evaluate a modified cytomorphological classification. This classification, an adaptation of a previously published system, categorised tumours based on cell shape (plasmacytic versus lymphocytic), but no association with chemoresistance was found (<jats:italic>p<\/jats:italic>\u2009=\u20090.083). Subsequently, a novel cytology scoring system was developed and tested to identify cases potentially associated with chemoresistance. This system was based on three criteria: anisokaryosis, mitotic count, and the presence of binucleated cells. Malignancy criteria, evaluated in 5 hotspots, were inversely associated with chemoresistance (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), predicted by low anisokaryosis, a mitotic count of \u2264\u20096 in a 2.37\u2009mm<jats:sup>2<\/jats:sup> area, and no binucleated cells. This study introduces a novel cytology scoring system for CTVTs, designed to serve as a predictive tool for vincristine treatment response. This system has the potential to aid practitioners in clinical decision\u2010making. However, further studies are required to validate its reliability and applicability.<\/jats:p>","DOI":"10.1111\/vco.13054","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T23:16:40Z","timestamp":1743463000000},"page":"278-289","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chemoresistance Cytological Score for Canine Transmissible Venereal Tumour: A Proposed System of Predicting Vincristine Resistance"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2385-8416","authenticated-orcid":false,"given":"Pedro Ant\u00f4nio Bronhara","family":"Pimentel","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana P\u00e1dua","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isadora Maria S\u00e1tiro","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphology Biological Sciences Institute, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayisa Rodrigues","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Ricardo Oliveira","family":"Paes","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayla Watanabe","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Stefanie Fonseca","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo dos Santos","family":"Horta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery Veterinary School, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-014-0168-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4336\/2023.pfb.43e202202262"},{"key":"e_1_2_10_4_1","first-page":"204","article-title":"Fine Needle Aspiration Cytology in the Diagnosis of Canine Cutaneous Transmissible Venereal Tumor\u2010Case Report","volume":"4","author":"Rocha N. 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