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As the most prevalent spontaneous tumour in canines, this disease model holds significant translational value for human non\u2010Hodgkin lymphoma research. To address diagnostic limitations in canine B\u2010cell lymphoma, we developed a canine\u2010specific CD19 monoclonal antibody (HAC19.1) with high affinity and established a dual\u2010platform detection system compatible with flow cytometry and immunohistochemistry. Additionally, a novel CD19\u2010targeting chimeric antigen receptor (CAR) gene sequence (HUA\u20101) was engineered and successfully transduced into Jurkat cells via lentiviral vectors, confirming stable CAR membrane expression. This breakthrough provides critical technical groundwork for advancing autologous CAR\u2010T cell therapy in canines.<\/jats:p>","DOI":"10.1111\/vco.70061","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T08:35:26Z","timestamp":1773822926000},"page":"370-381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation of a Rabbit Anti\u2010Canine\n                    <scp>CD19<\/scp>\n                    Monoclonal Antibody From Peripheral Blood and Its Validation in Immunoassays and\n                    <scp>CAR<\/scp>\n                    \u2010T Feasibility"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4864-152X","authenticated-orcid":false,"given":"Huixin","family":"Li","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiqi","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiting","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengjuan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengke","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan University of Animal Husbandry and Economy  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingda","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"},{"name":"Research Center for Organoids, College of Veterinary Medicine Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-21-0626"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2017.01.010"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mucimm.2024.07.005"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.55730\/1300-0144.5686"},{"key":"e_1_2_11_6_1","first-page":"696","article-title":"Impact of Prior CAR\u2010T Cell Therapy on Mosunetuzumab Efficacy in Patients With Relapsed or Refractory B\u2010Cell Lymphomas","volume":"4","author":"Chong E. 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