{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T19:37:05Z","timestamp":1786390625555,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K14087"],"award-info":[{"award-number":["JP23K14087"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25K09422"],"award-info":[{"award-number":["JP25K09422"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Canine large\u2010cell alimentary lymphoma (LCAL) is an aggressive malignancy with limited effective treatment options and a poor prognosis. In a preliminary drug screening study using three canine LCAL cell lines (CLC, Ema, and Nody\u20101), the taxane cabazitaxel (CBZ) was identified as a promising candidate drug. This study aimed to evaluate the antitumour effects of CBZ in\u00a0vitro and in\u00a0vivo and determine its potential as a novel therapeutic candidate for canine LCAL. Cell viability, tubulin polymerisation, cell cycle distribution, and apoptosis were assessed in CLC, Ema, and Nody\u20101 cells following treatment with CBZ or vehicle control. In addition, the effect of CBZ was evaluated in a xenotransplantation mouse model established using CLC cells. CBZ reduced cell viability across all cell lines in a dose\u2010dependent manner, with sub\u2010nanomolar half\u2010maximal inhibitory concentrations. A tubulin polymerisation assay demonstrated that CBZ increased polymerised tubulin levels, indicating microtubule stabilisation. CBZ treatment induced G2\/M cell cycle arrest and significantly increased apoptotic cell populations, accompanied by decreased procaspase\u20103 levels and increased cleaved caspase\u20103 expression. In\u00a0vivo, CBZ treatment reduced ascites accumulation and significantly prolonged survival relative to the vehicle control. These findings suggest that CBZ induces G2\/M cell cycle arrest and caspase\u2010dependent apoptosis through tubulin stabilisation and suppresses tumour progression in\u00a0vivo. CBZ represents a promising therapeutic candidate for canine LCAL.<\/jats:p>","DOI":"10.1111\/vco.70080","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T02:00:58Z","timestamp":1780365658000},"page":"593-600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cabazitaxel Demonstrates Potent Antitumour Activity Against Canine Large\u2010Cell Alimentary Lymphoma In\u00a0Vitro and In\u00a0Vivo"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9357-9394","authenticated-orcid":false,"given":"Kosei","family":"Sakai","sequence":"first","affiliation":[{"name":"Laboratory of Small Animal Internal Medicine 1, School of Veterinary Medicine Kitasato University  Towada Aomori Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory of Small Animal Internal Medicine 1, School of Veterinary Medicine Kitasato University  Towada Aomori Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Furuya","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, Graduate School of Veterinary Science Osaka Metropolitan University  Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ohta","sequence":"additional","affiliation":[{"name":"Companion Animal Internal Medicine, Department of Companion Animal Clinical Sciences, School of Veterinary Medicine Rakuno Gakuen University  Ebetsu Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kameshima","sequence":"additional","affiliation":[{"name":"Laboratory of Small Animal Internal Medicine 1, School of Veterinary Medicine Kitasato University  Towada Aomori Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Shimamura","sequence":"additional","affiliation":[{"name":"Laboratory of Small Animal Clinical Medicine, Graduate School of Veterinary Science Osaka Metropolitan University  Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"608","volume-title":"Withrow & Macewen's Small Animal Clinical Oncology","author":"Vail D. 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