{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T19:37:03Z","timestamp":1786390623270,"version":"build-2736575974"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Canine cutaneous mast cell tumours (MCTs) demonstrate variable clinical behaviour. Histological grading remains the primary prognostic approach, but some low\u2010grade tumours behave aggressively, and interobserver variability persists. This underscores the need for complementary and objective biomarkers. Raman spectroscopy (RS) is a non\u2010destructive optical technique based on the inelastic scattering of monochromatic light capable of detecting subtle biochemical changes in tissues. This exploratory study evaluates whether RS could differentiate low\u2010grade from high\u2010grade cutaneous MCTs based on characteristic vibrational signatures. Eighteen canine cutaneous mast cell tumours were graded according to the Kiupel two\u2010tier system, comprising 10 low\u2010grade and 8 high\u2010grade cases. Formalin\u2010fixed tumour samples were analysed, yielding a total of 68 Raman spectra. Spectra were acquired with a 785\u2009nm laser, pre\u2010processed using baseline correction, and normalized to the 1007\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    phenylalanine band. Data normality was tested with the Shapiro\u2013Wilk test, and group comparisons used the Mann\u2013Whitney\n                    <jats:italic>U<\/jats:italic>\n                    test (\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2009=\u20090.05). The 1007\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    band showed no significant difference between Low\u2010 and High\u2010Grade tumours (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.068), validating its use for normalization. Conversely, the 860\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    tyrosine\u2010associated band was significantly higher in high\u2010grade tumours (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.033). Ratios normalized to the 1007\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    peak consistently discriminated between grades, with significantly higher values in low\u2010grade tumours across multiple spectral regions\u2014particularly 1007\/1299 and 1007\/1301\u20131320 (all\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Overall, RS identified distinct biochemical fingerprints between tumour grades in canine cutaneous MCTs, especially in spectral regions associated with amino acids, lipids, and nucleic acids. These findings support the potential of RS as a complementary tool for MCT characterization and encourage further validation in larger cohorts with clinical outcome data.\n                  <\/jats:p>","DOI":"10.1111\/vco.70083","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:56:42Z","timestamp":1782262602000},"page":"621-630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raman Spectroscopy for Differentiating High\u2010 and Low\u2010Grade Canine Cutaneous Mast Cell Tumours"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9330-8746","authenticated-orcid":false,"given":"Jos\u00e9","family":"Catarino","sequence":"first","affiliation":[{"name":"I\u2010MVet, Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona\u2010Centro Universit\u00e1rio de Lisboa  Lisboa Portugal"},{"name":"Comprehensive Health Research Centre (CHRC) &amp; Departamento de Medicina Veterin\u00e1ria Escola de Ci\u00eancia e Tecnologia, Universidade de \u00c9vora  \u00c9vora Portugal"},{"name":"IPLUSO, Instituto Polit\u00e9cnico da Lusofonia  Lisboa Portugal"},{"name":"DNAtech, Laborat\u00f3rio Veterin\u00e1rio  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Silva","sequence":"additional","affiliation":[{"name":"INESC TEC Instituto de Engenharia de Sistemas e Computadores Tecnologia e Ci\u00eancia  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Novais","sequence":"additional","affiliation":[{"name":"INESC TEC Instituto de Engenharia de Sistemas e Computadores Tecnologia e Ci\u00eancia  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Santos","sequence":"additional","affiliation":[{"name":"I\u2010MVet, Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona\u2010Centro Universit\u00e1rio de Lisboa  Lisboa Portugal"},{"name":"DNAtech, Laborat\u00f3rio Veterin\u00e1rio  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5225-4510","authenticated-orcid":false,"given":"Rita","family":"Payan\u2010Carreira","sequence":"additional","affiliation":[{"name":"Comprehensive Health Research Centre (CHRC) &amp; Departamento de Medicina Veterin\u00e1ria Escola de Ci\u00eancia e Tecnologia, Universidade de \u00c9vora  \u00c9vora Portugal"},{"name":"Departamento de Medicina Veterin\u00e1ria Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora  \u00c9vora Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3922-3602","authenticated-orcid":false,"given":"Pedro","family":"Fa\u00edsca","sequence":"additional","affiliation":[{"name":"I\u2010MVet, Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona\u2010Centro Universit\u00e1rio de Lisboa  Lisboa Portugal"},{"name":"DNAtech, Laborat\u00f3rio Veterin\u00e1rio  Lisboa Portugal"},{"name":"CECAV\u2010 Centro de Ci\u00eancia Animal e Veterin\u00e1ria\u2010 Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona\u2010Centro Universit\u00e1rio de Lisboa  Portugal"},{"name":"CBIOS \u2010 Centro de Investiga\u00e7\u00e3o Em Bioci\u00eancias e Tecnologias da Sa\u00fade \u2010 Universidade Lus\u00f3fona  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Reis","sequence":"additional","affiliation":[{"name":"CISAS &amp; ESA, IPVC \u2010 Instituto Polit\u00e9cnico de Viana do Castelo  Viana do Castelo Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12581"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/03009858251315094"},{"key":"e_1_2_10_4_1","first-page":"236","article-title":"Exploring Canine Mast Cell Tumors: An Investigation Into Demographic Characteristics, and Grading System Analysis From a Pathology Lab Data (2019\u20132021)","volume":"1","author":"Catarino J.","year":"2025","journal-title":"Preventive Veterinary Medicine"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2019.04.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.239.7.960"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.755258"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810386469"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985814521638"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098588402100503"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985820985138"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985819890686"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985820973463"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9090493"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711425945"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985817747325"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.07.006"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.05622"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44-3-298"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.11.018"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3658756"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10091652"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-018-9770-9"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914748"},{"issue":"1","key":"e_1_2_10_25_1","first-page":"84","article-title":"Clinical and Investigative Applications of Raman Spectroscopy in Urology and Andrology","volume":"3","author":"Osterberg E. 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