{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T19:37:04Z","timestamp":1786390624987,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K21375"],"award-info":[{"award-number":["20K21375"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H02366"],"award-info":[{"award-number":["21H02366"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Canine mammary gland tumours (MGT) represent a common malignancy in intact female dogs, with a high metastasis rate approaching 50% and a poor prognosis. However, metastasis\u2010related genes remain largely unelucidated. Accordingly, we aimed to identify differentially expressed mRNAs and miRNAs in primary canine MGT tissues from dogs with or without confirmed metastasis. We compared mRNA and microRNA (miRNA) expression profiles between malignant MGT tissue samples with and without metastasis. Differentially expressed genes and miRNAs were identified using next\u2010generation sequencing and analysed using the Empirical Analysis of DEGs tool within CLC Genomics Workbench. We identified 119 genes and eight miRNAs as differentially expressed in primary MGTs from dogs with confirmed metastasis. The RIPPLY1 gene was significantly downregulated in primary MGT samples with confirmed metastasis and this result was validated by RT\u2010qPCR. The Cancer Genome Atlas (TCGA) database showed decreased RIPPLY1 expression in human breast cancer, supporting its possible relevance to tumour biology. In silico analysis (miRWalk) revealed that miR\u2010187 is a predicted candidate miRNA that may interact with RIPPLY1 and both NGS data and RT\u2010qPCR showed increased cfa\u2010miR\u2010187 expression in primary tumour samples from dogs with confirmed metastasis. The inverse expression pattern of RIPPLY1 and cfa\u2010miR\u2010187, together with RNAhybrid binding prediction, suggests a possible candidate miRNA\u2013mRNA relationship. STRING analysis suggested a possible pathway context involving beta\u2010catenin\/TCF\u2010related proteins, but this computational result requires further validation. Based on these findings, we identified RIPPLY1 and cfa\u2010miR\u2010187 as potential candidate transcripts associated with primary canine MGTs from dogs with confirmed metastasis but functional validation is required.<\/jats:p>","DOI":"10.1111\/vco.70084","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:16:06Z","timestamp":1783062966000},"page":"631-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic and\n                    <scp>microRNA<\/scp>\n                    Profiling Suggest a Candidate\n                    <scp>RIPPLY1<\/scp>\n                    \u2013cfa\u2010\n                    <scp>miR<\/scp>\n                    \u2010187 Relationship in Canine Mammary Gland Tumours From Dogs With Confirmed Metastasis"],"prefix":"10.1111","volume":"24","author":[{"given":"Shaohsu","family":"Wang","sequence":"first","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Shi Kagoshima Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4603-5594","authenticated-orcid":false,"given":"Masashi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Shi Kagoshima Ken Japan"},{"name":"Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Shi Kagoshima Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Shi Kagoshima Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiro","family":"Nozaki","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  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