{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T10:15:38Z","timestamp":1783332938231,"version":"3.54.6"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972743"],"award-info":[{"award-number":["31972743"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2024NSFSC0372"],"award-info":[{"award-number":["2024NSFSC0372"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Triple negative breast cancer (TNBC) presents a significant challenge in breast cancer treatment. Canine mammary carcinoma, characterized by spontaneous tumours in female dogs, serves as a robust spontaneous model for TNBC. In this study, DPIA, a potent antitumor agent, was encapsulated into synthesized mPEG\u2010PCL (mPP) to form nano\u2010micelles, aiming to enhance its in\u00a0vivo therapeutic efficacy. DPIA@mPP exhibited more uniform dispersion and a spheroid\u2010like architecture, with a drug loading rate of 4.31% and an encapsulation rate of 13.10%. Both DPIA@mPP and free DPIA exhibited comparable and significant cytotoxicity against canine mammary carcinoma cells (CMT\u20107364) in\u00a0vitro. Encapsulation into mPP enhanced DPIA uptake by CMT\u20107364 cells, increasing tumour inhibition from 30.31% to 48.55%, while markedly reducing in\u00a0vivo toxicity. Overall, the DPIA@mPP group demonstrated superior in\u00a0vivo antitumor efficacy and a good safety profile. Furthermore, preliminary mechanistic studies suggest that DPIA@mPP may promote apoptosis in CMT\u20107364 cells, potentially through the inhibition of the PI3K\/AKT signalling pathway, offering novel insights into TNBC treatment strategies.<\/jats:p>","DOI":"10.1111\/vco.70086","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T09:34:09Z","timestamp":1783330449000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010Canine Mammary Carcinoma Effects of\n                    <scp>mPP<\/scp>\n                    \u2010Based Nano\u2010Micelles Encapsulating\n                    <scp>DPIA<\/scp>"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8280-6218","authenticated-orcid":false,"given":"Yuhong","family":"Chen","sequence":"first","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Funeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Liang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, Department of Pharmacy Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Agricultural Bioinformatics, Ministry of Education Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"issue":"11","key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/cells9112358","article-title":"Estrogen Actions in Triple\u2010Negative Breast Cancer","volume":"9","author":"Treeck O.","year":"2020","journal-title":"Cells"},{"issue":"2","key":"e_1_2_13_3_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s10549-016-4059-6","article-title":"Triple\u2010Negative Breast Cancer Has Worse Overall Survival and Cause\u2010Specific Survival Than Non\u2010Triple\u2010Negative Breast Cancer","volume":"161","author":"Li X.","year":"2017","journal-title":"Breast Cancer Research and Treatment"},{"issue":"3","key":"e_1_2_13_4_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1158\/1078-0432.CCR-16-0162","article-title":"Efficacy of Neoadjuvant Carboplatin Plus Docetaxel in Triple\u2010Negative Breast Cancer: Combined Analysis of Two Cohorts","volume":"23","author":"Sharma P.","year":"2017","journal-title":"Clinical Cancer Research"},{"key":"e_1_2_13_5_1","doi-asserted-by":"crossref","first-page":"623800","DOI":"10.3389\/fvets.2021.623800","article-title":"From Conventional to Precision Therapy in Canine Mammary Cancer: A Comprehensive Review","volume":"8","author":"Valdivia G.","year":"2021","journal-title":"Frontiers in Veterinary Science"},{"issue":"5","key":"e_1_2_13_6_1","first-page":"127","article-title":"Epidemiological Study of Mammary Tumors in Female Dogs Diagnosed During the Period 2002\u20102012: A Growing Animal Health Problem","volume":"10","author":"Seagroves T.","year":"2015","journal-title":"PLoS One"},{"issue":"3","key":"e_1_2_13_7_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1007\/s10549-017-4548-2","article-title":"Canine Invasive Mammary Carcinomas as Models of Human Breast Cancer. Part 1: Natural History and Prognostic Factors","volume":"167","author":"Nguyen F.","year":"2017","journal-title":"Breast Cancer Research and Treatment"},{"issue":"6","key":"e_1_2_13_8_1","first-page":"8195","article-title":"Canine Mammary Tumors as a Model for Human Disease (Review)","volume":"15","author":"Abdelmegeed S.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_13_9_1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.3389\/fonc.2020.00617","article-title":"Naturally\u2010Occurring Canine Mammary Tumors as a Translational Model for Human Breast Cancer","volume":"10","author":"Gray M.","year":"2020","journal-title":"Frontiers in Oncology"},{"key":"e_1_2_13_10_1","doi-asserted-by":"crossref","first-page":"563779","DOI":"10.3389\/fonc.2020.563779","article-title":"Triple\u2010Negative Breast Cancer Comparison With Canine Mammary Tumors From Light Microscopy to Molecular Pathology","volume":"10","author":"Namagerdi A. 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