{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:55:05Z","timestamp":1792050905487,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Vet Comparative Oncology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Feline mammary carcinoma (FMC) is an aggressive and highly metastatic cancer that shares molecular and clinical characteristics with human triple\u2010negative breast cancer (TNBC), making it a relevant comparative model for a disease lacking targeted therapies. This study investigated the feasibility and clinical utility of\n                    <jats:sup>64<\/jats:sup>\n                    CuCl\n                    <jats:sub>2<\/jats:sub>\n                    PET\/CT imaging in cats with naturally occurring mammary carcinoma. Eight cats (seven tumour\u2010bearing, one healthy control) were administered an intravenous dose of\n                    <jats:sup>64<\/jats:sup>\n                    CuCl\n                    <jats:sub>2<\/jats:sub>\n                    and underwent whole\u2010body PET\/CT imaging. Semi\u2010quantitative analysis was performed using standardized uptake values (SUVs), and tumour\u2010to\u2010muscle ratios were calculated to assess lesion conspicuity. Serum ceruloplasmin concentrations were measured as a marker of systemic copper metabolism. All primary masses were\n                    <jats:sup>64<\/jats:sup>\n                    Cu\u2010avid and conspicuous, with consistently elevated tumour\u2010to\u2010muscle ratios across tumour\u2010bearing cats, allowing clear differentiation between neoplastic and normal tissue. Imaging provided additional staging information by identifying locally invasive and metastatic disease that was not fully evident on physical examination. Physiologic tracer distribution was characterized by high uptake in the liver and gallbladder and low uptake in skeletal muscle, which served as a reliable reference tissue. Serum ceruloplasmin concentrations varied widely and did not correlate with tumour uptake. These findings suggest that\n                    <jats:sup>64<\/jats:sup>\n                    CuCl\n                    <jats:sub>2<\/jats:sub>\n                    PET\/CT is a feasible functional imaging modality for FMC, potentially aiding in tumour detection. This was an exploratory study, and not all imaging results could be confirmed by cytology or histopathology. Nonetheless, our findings suggest that\n                    <jats:sup>64<\/jats:sup>\n                    CuCl\n                    <jats:sub>2<\/jats:sub>\n                    PET\/CT should be investigated further as a method for probing copper utilization in FMC.\n                  <\/jats:p>","DOI":"10.1111\/vco.70125","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:35:46Z","timestamp":1792049746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A New Application of\n                    <scp>\n                      <sup>64<\/sup>\n                      CuCl\n                      <sub>2<\/sub>\n                      PET\n                    <\/scp>\n                    \/\n                    <scp>CT<\/scp>\n                    : Imaging of Cats With Mammary Carcinoma"],"prefix":"10.1111","author":[{"given":"Vivan A.","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joni M.","family":"Lunceford","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7471-184X","authenticated-orcid":false,"given":"Colleen","family":"Garrett","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9981-2903","authenticated-orcid":false,"given":"Jimmy C.","family":"Lattimer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Papageorgiou","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7330-4185","authenticated-orcid":false,"given":"Charles J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Research Service Harry S. Truman Memorial Veterans' Hospital  Columbia Missouri USA"},{"name":"Research Reactor Center, University of Missouri  Columbia Missouri USA"},{"name":"Department of Radiology University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2075-3926","authenticated-orcid":false,"given":"Charles A.","family":"Maitz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"},{"name":"Ellis Fischel Cancer Center, University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6820-9850","authenticated-orcid":false,"given":"Jeffrey N.","family":"Bryan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"},{"name":"Ellis Fischel Cancer Center, University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica L.","family":"Talbott","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1322-6346","authenticated-orcid":false,"given":"Michael R.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery University of Missouri  Columbia Missouri USA"},{"name":"Ellis Fischel Cancer Center, University of Missouri  Columbia Missouri USA"},{"name":"Molecular Imaging and Theranostics Center, University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"604","volume-title":"Withrow and Macewen's Small Animal Clinical Oncology","author":"Sorenmo K. U.","year":"2020"},{"key":"e_1_2_9_3_1","first-page":"307","article-title":"Survey of Animal Neoplasms in Alameda and Contra Costa Counties, California. II. Cancer Morbidity in Dogs and Cats From Alameda County","volume":"40","author":"Dorn C. R.","year":"1968","journal-title":"Journal of the National Cancer Institute"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1177\/0300985814528221","article-title":"Prognostic Evaluation of Feline Mammary Carcinomas","volume":"52","author":"Zappulli V.","year":"2015","journal-title":"Veterinary Pathology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"105775","DOI":"10.18632\/oncotarget.22398","article-title":"Serum SDF\u20101 Levels Are a Reliable Diagnostic Marker of Feline Mammary Carcinoma","volume":"8","author":"Marques C. S.","year":"2017","journal-title":"Oncotarget"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3390\/vetsci12080736","article-title":"Feline Mammary Tumors: A Comprehensive Review","volume":"12","author":"Rodrigues\u2010Jesus J.","year":"2025","journal-title":"Veterinary Sciences"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1177\/1098612X15610367","article-title":"Evaluation of Prognostic Factors and Survival Rates in Malignant Feline Mammary Gland Neoplasms","volume":"18","author":"De Campos C. B.","year":"2016","journal-title":"Journal of Feline Medicine and Surgery"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.rvsc.2025.105789","article-title":"Lymph Node Status of Felines Affected by Mammary Gland Neoplasms: A Look Beyond the Presence or Absence of Metastasis","volume":"193","author":"Souza F. R.","year":"2025","journal-title":"Research in Veterinary Science"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"779","DOI":"10.3390\/ani15060779","article-title":"Prognostic Insights in Feline Mammary Carcinoma","volume":"15","author":"Monteiro M.","year":"2025","journal-title":"Animals"},{"key":"e_1_2_9_10_1","volume-title":"Classification of Tumours in Domestic Animals","author":"Owen L. N.","year":"1980"},{"key":"e_1_2_9_11_1","first-page":"4053","article-title":"St Gallen Molecular Subtypes in Feline Mammary Carcinoma","volume":"37","author":"Soares M.","year":"2015","journal-title":"Tumour Biology"},{"key":"e_1_2_9_12_1","article-title":"Feline Mammary Basal\u2010Like Adenocarcinomas","volume":"13","author":"Wiese D. A.","year":"2013","journal-title":"BMC Cancer"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.3892\/ijo.2020.5135","article-title":"Triple\u2010Negative Breast Cancer Therapy: Current and Future Perspectives","volume":"57","author":"Won K.\u2010A.","year":"2020","journal-title":"International Journal of Oncology"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/s41568-021-00417-2","article-title":"Connecting Copper and Cancer: From Transition Metal Signaling to Metalloplasisa","volume":"22","author":"Ge E. J.","year":"2022","journal-title":"Nature Reviews. Cancer"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2023.114791","article-title":"Copper in Cancer: From Pathogenesis to Therapy","volume":"163","author":"Guang D.","year":"2023","journal-title":"Biomedicine & Pharmacotherapy"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","DOI":"10.3390\/cancers12123594","article-title":"The Multifaceted Roles of Copper in Cancer","volume":"12","author":"Leli\u00e8vre P.","year":"2020","journal-title":"Cancers"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1128\/MCB.05722-11","article-title":"A Novel Role for Copper in Ras\/Mitogen\u2010Activated Protein Kinase Signaling","volume":"32","author":"Turski M. L.","year":"2012","journal-title":"Molecular and Cellular Biology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1177\/1534735402238185","article-title":"Cancer Therapy With Tetrathiomolybdate: Antiangiogenesis by Lowering Body Copper","volume":"1","author":"Brewer G. J.","year":"2002","journal-title":"Integrative Cancer Therapies"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s13139-022-00738-6","article-title":"Recent Advances in Cancer Imaging With 64CuCl2 PET\/CT","volume":"56","author":"Peng F.","year":"2022","journal-title":"Nuclear Medicine and Molecular Imaging"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","DOI":"10.3390\/jcm12010223","article-title":"Targeting Copper in Cancer Imaging and Therapy","volume":"12","author":"Capriotti G.","year":"2023","journal-title":"Journal of Clinical Medicine"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-27559-z","article-title":"Copper Depletion Modulates Mitochondrial Oxidative Phosphorylation","volume":"12","author":"Ramchandani D.","year":"2021","journal-title":"Nature Communications"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1089\/cbr.2009.0674","article-title":"Copper\u201064 Radiopharmaceuticals for PET Imaging of Cancer: Advances in Preclinical and Clinical Research","volume":"24","author":"Anderson C. J.","year":"2009","journal-title":"Cancer Biotherapy & Radiopharmaceuticals"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.nucmedbio.2025.109596","article-title":"PET Imaging of [64Cu]Copper(II) Chloride and Tetrathiomolybdate Administration in Animal Models of Triple\u2010Negative Breast Cancer","volume":"152","author":"Lewis M. R.","year":"2026","journal-title":"Nuclear Medicine and Biology"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1158\/2767-9764.CRC-26-0036","article-title":"Tumor CTR1 Expression and Systemic Copper Dynamics Converge on a Copper Axis in High\u2010Grade Triple\u2010Negative Breast Cancer","volume":"6","author":"Shanbhag V.","year":"2026","journal-title":"Cancer Research Communications"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.70125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vco.70125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vco.70125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:35:50Z","timestamp":1792049750000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vco.70125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,15]]},"references-count":23,"alternative-id":["10.1111\/vco.70125"],"URL":"https:\/\/doi.org\/10.1111\/vco.70125","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"value":"1476-5810","type":"print"},{"value":"1476-5829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,15]]},"assertion":[{"value":"2026-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"vco.70125"}}