{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:20:04Z","timestamp":1790040004279,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T00:00:00Z","timestamp":1356912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A recent genome\u2010wide scan using the canine minimal screening set 2 (<jats:styled-content style=\"fixed-case\">MSS<\/jats:styled-content>\u20102) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers<jats:styled-content style=\"fixed-case\">DTR<\/jats:styled-content>13.6 and<jats:styled-content style=\"fixed-case\">REN<\/jats:styled-content>13N11 on canine chromosome 13.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The goal of this study was to evaluate the<jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>_<jats:styled-content style=\"fixed-case\">CANFA<\/jats:styled-content>gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker<jats:styled-content style=\"fixed-case\">DTR<\/jats:styled-content>13.6, for any mutations in this breed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the<jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>_<jats:styled-content style=\"fixed-case\">CANFA<\/jats:styled-content>gene. The genotype and gene sequence were compared between cobalamin\u2010deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>sequences published as part of the Ensemble Genomic map.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neither the microsatellite markers (Myc and G15987) nor the sequences of the<jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>_<jats:styled-content style=\"fixed-case\">CANFA<\/jats:styled-content>gene showed a significant difference among both groups of Shar Peis and the published canine<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the<jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>_<jats:styled-content style=\"fixed-case\">CANFA<\/jats:styled-content>gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to<jats:styled-content style=\"fixed-case\">DTR<\/jats:styled-content>13.6 and<jats:styled-content style=\"fixed-case\">REN<\/jats:styled-content>13N11 that shows mutations in cobalamin\u2010deficient Shar Peis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12010","type":"journal-article","created":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T20:19:51Z","timestamp":1357071591000},"page":"61-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the<scp>MYC<\/scp>_<scp>CANFA<\/scp>gene in Chinese Shar Peis with cobalamin deficiency"],"prefix":"10.1111","volume":"42","author":[{"given":"Niels","family":"Gr\u00fctzner","sequence":"first","affiliation":[{"name":"Gastrointestinal Laboratory Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micah A.","family":"Bishop","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan S.","family":"Suchodolski","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,31]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.05.008"},{"key":"e_1_2_3_3_1","first-page":"722","article-title":"Breed predispositions for severe hypocobalaminemia and relation to folate concentration in dogs with gastrointestinal disease","volume":"24","author":"Dandrieux JRS","year":"2010","journal-title":"J Vet Intern Med"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/esp100"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01025-6"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.22.10217"},{"key":"e_1_2_3_7_1","doi-asserted-by":"crossref","first-page":"940","DOI":"10.2460\/ajvr.1991.52.06.940","article-title":"Proto\u2010oncogenes of genomic DNA in clinically normal animals of various species","volume":"52","author":"Miyoshi N","year":"1991","journal-title":"Am J Vet Res"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8394"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.10.2711.bloodjournal85102711"},{"key":"e_1_2_3_10_1","first-page":"191","article-title":"Serum transcobalamin II levels in patients with acute and chronic renal failure","volume":"78","author":"Areekul S","year":"1995","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.1992.tb00972.x"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01955004"},{"key":"e_1_2_3_13_1","first-page":"549","article-title":"Inherited transcobalamin II deficiency: clinical, genetic studies and diagnosis using cultured fibroblasts","volume":"38","author":"Sacher M","year":"1983","journal-title":"Helv Paediatr Acta"},{"key":"e_1_2_3_14_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1182\/blood.V63.3.598.598","article-title":"Congenital transcobalamin II deficiency presenting atypically with a low serum cobalamin level\u2010studies demonstrating the coexistence of a circulating transcobalamin\u2010I (R binder) complex","volume":"63","author":"Carmel R","year":"1984","journal-title":"Blood"},{"key":"e_1_2_3_15_1","unstructured":"NCBI map database: Canis familiaris (CanFam 2.0). 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