{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:25:50Z","timestamp":1761863150242},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T00:00:00Z","timestamp":1356912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The presence of human breast carcinoma micrometastases in bone marrow is associated with poor overall survival, poor breast\u2010cancer\u2010specific survival, poor disease\u2010free survival, and poor distant disease\u2010free survival. In veterinary practice, the detection of micrometastases as a component of clinical staging is a routine practice for lymphomas and mast cell tumors, but not for carcinomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This prospective study evaluated whether the identification of micrometastases from various carcinomas in dogs and cats in bone marrow using cell block cytology is technically feasible and whether it correlates with routine cytologic examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirteen dogs and 4 cats with various types of carcinomas were available for analysis. Routine and cell block cytologic evaluation combined with immunocytochemical staining with antibodies to <jats:styled-content style=\"fixed-case\">CKAE<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>7 were performed on all bone marrow samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bone marrow micrometastasis was demonstrated by both methods in 2 dogs with advanced disease. In one case cells were immunoreactive for both <jats:styled-content style=\"fixed-case\">CKAE<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates that cell block cytology is a practical and useful method for bone marrow evaluation and is suitable for cytokeratin immunocytochemical analysis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12011","type":"journal-article","created":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T20:19:51Z","timestamp":1357071591000},"page":"85-91","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Detection of carcinoma micrometastases in bone marrow of dogs and cats using conventional and cell block cytology"],"prefix":"10.1111","volume":"42","author":[{"given":"Bonnie E.","family":"Taylor","sequence":"first","affiliation":[{"name":"Donaldson\u2010Atwood Cancer Center The Animal Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole F.","family":"Leibman","sequence":"additional","affiliation":[{"name":"Donaldson\u2010Atwood Cancer Center The Animal Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Luong","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine Stanford University School of Medicine Stanford CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew S.","family":"Loar","sequence":"additional","affiliation":[{"name":"ALX Laboratories New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane M.","family":"Craft","sequence":"additional","affiliation":[{"name":"Department of Pathology The Animal Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.09.027"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050434"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-2515"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-011-1379-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/G035-P2MM-D1TM-T5QE"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.28.7045"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.12.014"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.97"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2011.01.025"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2010.02584.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010021"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-010-0617-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.141"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2010.04.008"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-9-43"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00456.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.078410"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21457"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-010-0217-5"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2007.00477.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.5858\/2003-127-0579-CIICBP","article-title":"Cytokeratin 19 immunolocalization in cell block preparation of thyroid aspirates. 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