{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T06:10:09Z","timestamp":1696054209785},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T00:00:00Z","timestamp":1379635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>During pregnancy, glucocorticoids are frequently used to accelerate fetal lung maturation in preterm delivery. However, prenatal administration of glucocorticoids has been shown to affect organs such as fetal liver, an important hematopoietic organ during fetal development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to document the qualitative and quantitative changes in erythroid and megakaryocytic cell populations found in fetal livers as well as the hematology profile in neonates after maternal glucocorticoid treatment in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pregnant female Wistar rats were treated with dexamethasone 21\u2010phosphate from days 13 to 16 of gestation. On the 17th day of pregnancy, the fetuses were collected and their livers processed for light and transmission electron microscopy. Glycol methacrylate\u2010embedded sections were stained with <jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content> to determine the erythroblast and megakaryocytic cell frequencies. Fetal liver pieces embedded in Spurr resin were analyzed by transmission electron microscopy for morphologic changes. A standard hematology profile was evaluated in neonatal rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the fetuses from treated dams, the total cell number of erythroid cells in livers was significantly reduced compared to control fetuses (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001), but erythroblasts did not present ultrastructural abnormalities. The degree of maturation in the megakaryocyte series tended to be increased. In neonates, there were elevated numbers of nucleated <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.002), along with a higher <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HGB<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.02). In addition, the platelet concentration was also significantly increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.007).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that maternal dexamethasone treatment has quantitative effects on erythroid and megakaryocytic cells in fetal liver and the neonatal hematology profile in rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12080","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T23:58:56Z","timestamp":1381535936000},"page":"483-489","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Morphometric evaluation of the fetal rat liver after maternal dexamethasone treatment: effect on the maturation of erythroid and megakaryocytic cells"],"prefix":"10.1111","volume":"42","author":[{"given":"Fl\u00e1via Macedo de Oliveira","family":"Neves","sequence":"first","affiliation":[{"name":"Department of Morphology and Genetics Laboratory of Developmental Biology Federal University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Cicconi","family":"Paccola","sequence":"additional","affiliation":[{"name":"Department of Morphology and Genetics Laboratory of Developmental Biology Federal University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra Maria","family":"Miraglia","sequence":"additional","affiliation":[{"name":"Department of Morphology and Genetics Laboratory of Developmental Biology Federal University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivone","family":"Cipriano","sequence":"additional","affiliation":[{"name":"Department of Morphology and Genetics Laboratory of Developmental Biology Federal University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,20]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0272-0590(92)90093-W"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200301000-00017"},{"key":"e_1_2_3_4_1","first-page":"231","article-title":"Anatomy and embryology of the laboratory rat","author":"Hebel R","year":"1986","journal-title":"W\u00f6rthsee: BioMed Verlag"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.3.627"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e3283497ed5"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2009.07.006"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-077883"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.449"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.06.031"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2000.8904"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.2.267"},{"key":"e_1_2_3_13_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1182\/blood.V94.2.550","article-title":"The glucocorticoid receptor cooperates with the erythropoietin receptor and c\u2010Kit to enhance and sustain proliferation of erythroid progenitors in vitro","volume":"94","author":"Von Lindern M","year":"1999","journal-title":"Blood"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108269"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-03-1002"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1991.tb11827.x"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.104.3.476"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-85816-6_5"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00106.2004"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(83)90046-0"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000241375"},{"key":"e_1_2_3_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2009.02.005"},{"key":"e_1_2_3_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-1443"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12080","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:37:56Z","timestamp":1696023476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,20]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1111\/vcp.12080"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12080","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,20]]},"assertion":[{"value":"2013-09-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}