{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:47:31Z","timestamp":1763747251334},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,10,25]],"date-time":"2013-10-25T00:00:00Z","timestamp":1382659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May\u2013Gr\u00fcnwald\u2013Giemsa and the Toluidine Blue staining methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The cytologic specimens obtained from fine\u2010needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high\u2010power field (<jats:styled-content style=\"fixed-case\">HPF<\/jats:styled-content>, x40 objective). The ratio of mast cells\/100 hepatocytes was then compared between the 2 staining methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A median of 0.17 (range: 0\u20130.33) mast cells\/100 hepatocytes and of 0.47 (range: 0\u20130.86) mast cells\/100 hepatocytes was obtained with May\u2013Gr\u00fcnwald\u2013Giemsa and Toluidine Blue stains, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Toluidine Blue stain detects more hepatic mast cells than May\u2013Grunwald\u2013Giemsa in normal canine livers. With both stains, &lt; 1% mast cells were detected in normal canine liver samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12086","type":"journal-article","created":{"date-parts":[[2013,10,25]],"date-time":"2013-10-25T19:00:33Z","timestamp":1382727633000},"page":"522-525","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Proportion of mast cells in normal canine hepatic cytologic specimens: comparison of 2 staining methods"],"prefix":"10.1111","volume":"42","author":[{"given":"Carlo","family":"Masserdotti","sequence":"first","affiliation":[{"name":"San Marco Private Veterinary Laboratory Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,25]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.62.1183"},{"key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.4049\/jimmunol.154.4.1830","article-title":"IL\u20104 renders mast cells functionally responsive to endothelin\u20101","volume":"154","author":"Egger D","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_2_4_1","first-page":"5","article-title":"The mast cell integrates the splanchnic and systemic inflammatory response in portal hypertension","volume":"24","author":"Aller MA","year":"2007","journal-title":"J Transl Med"},{"key":"e_1_2_2_5_1","first-page":"203","article-title":"Relevance of mast cells and hepatic lobule innervation to liver injury","volume":"13","author":"Stoyanova II","year":"2004","journal-title":"Rom J Gastroenterol"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i8.1141"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i7.1469"},{"key":"e_1_2_2_8_1","first-page":"9","article-title":"Cytologic features of hepatic fibrosis in dogs: description of 11 cases","volume":"22","author":"Masserdotti C","year":"2008","journal-title":"Veterinaria"},{"key":"e_1_2_2_9_1","first-page":"227","volume-title":"Atlas of Canine and Feline Cytology","author":"Meyer DJ","year":"2001"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-1-154"},{"issue":"6","key":"e_1_2_2_11_1","first-page":"809","article-title":"Fine needle cytology\u2013is aspiration suction necessary? A study of 100 masses in various sites","volume":"33","author":"Mair S","year":"1989","journal-title":"Acta Cytol"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0354.x"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2006.00107.x"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600410"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520290902991394"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:HIJO.0000023377.70443.08"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:38:11Z","timestamp":1696023491000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,25]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1111\/vcp.12086"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12086","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,25]]},"assertion":[{"value":"2013-10-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}