{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T15:53:29Z","timestamp":1769356409067,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T00:00:00Z","timestamp":1386547200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 4\u2010year\u2010old neutered female crossbred Shepherd was referred for a history of 10\u00a0days of anorexia, polyuria, polydipsia, polyadenomegaly, and diarrhea. On physical examination, the dog appeared quiet, responsive, and apyretic, with generalized and severe lymphadenomegaly. Hematologic abnormalities included neutrophilic leukocytosis with left shift, and lymphopenia. Blood smears revealed intracytoplasmic bacilli negatively stained with May\u2013Gr\u00fcnwald\u2013Giemsa in neutrophils and monocytes. Lymph node smears revealed pyogranulomatous adenitis with calcified deposits and many negative\u2010staining rod structures, both within the cytoplasm of neutrophils and macrophages, and free in the background. An acid\u2010fast stain (Ziehl\u2013Neelsen) confirmed the diagnosis of mycobacterial infection. The dog was euthanized for public health and ethical reasons, and the postmortem examination revealed severe and generalized granulomatous and necrotizing lymphadenitis, panniculitis, and hepatitis, and infiltration of epithelioid macrophages in the lungs, colon, and spleen. Numerous acid\u2010fast bacilli, consistent with mycobacterial infection, were observed both in the cytoplasm of epithelioid macrophages and giant cells, and free in the background.<jats:italic>Mycobacterium bovis<\/jats:italic>was first confirmed by conventional<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>of organ extracts.<jats:italic>Mycobacterium avium<\/jats:italic>was detected in a culture of the same organs. Further<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>amplifications and sequencing revealed a coinfection with 2 different species of mycobacterium, one belonging to the<jats:italic>Mycobacterium avium<\/jats:italic>complex and the other to the<jats:italic>Mycobacterium tuberculosis<\/jats:italic>complex.<\/jats:p>","DOI":"10.1111\/vcp.12088","type":"journal-article","created":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T16:53:50Z","timestamp":1386608030000},"page":"516-521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A mycobacterial coinfection in a dog suspected on blood smear"],"prefix":"10.1111","volume":"42","author":[{"given":"Claire\u2010Lise","family":"Etienne","sequence":"first","affiliation":[{"name":"Department of Pathology Universit\u00e9 de Toulouse INP ENVT Toulouse 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